The eyeballs and eyelids collected from mice were fixed in 4% paraformaldehyde, then embedded with paraffin and sectioned into 5 µm thickness. A PAS staining kit (Servicebio, Wuhan, China) was used to stain the sections. All sections were observed and photographed with a digital light microscope (Nikon, Tokyo, Japan).
Digital light microscope
Manufactured by Nikon
Sourced in Japan
The Digital Light Microscope is a laboratory instrument designed for magnifying and observing small objects with the use of light. It captures digital images of the magnified specimens.
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Lab products found in correlation
4 protocols using digital light microscope
1
PAS Staining of Mouse Eyeballs
2
Histological Analysis of Mouse Conjunctiva
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The eyeballs were collected, fixed with 4% paraformaldehyde (PFA), paraffin embedded, sectioned at a 5 μm thickness, and stained with PAS staining kit (Cat#1008, Servicebio, Wuhan, China) according to the manufactory’s instruction. The superior and inferior conjunctiva were examined and photographed with a digital light microscope (Nikon). Three sections from the central parts of the eye from each animal were studied. Six mice were used for each group.
3
Histological Analysis of Spinal Cord
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For histological analysis, the spinal cord was harvested at the peak of EAE. The tissue was prepared for paraffin embedding and sectioning (8 μm thick). The sections were deparaffinized, rehydrated, and stained with Luxol Fast Blue (LFB) following standard immune cell infiltration or demyelination analysis procedures. Staining was evaluated using a Nikon digital light microscope (Tokyo, Japan).
4
Histological Examination of Organ Tissues
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Firstly, liver, spleen and kidney tissues were dissected, fixed in 10% (v/v) formalin solution, embedded in a paraffin box and cut using a microtome. Secondly, the tissue sections were deparaffinized and rehydrated sequentially in xylene, 100% (v/v) ethanol, 95% ethanol, 80% ethanol and DI. Thirdly, the sections were stained with hematoxylin solution (Harris hematoxylin combined with glacial acetic acid), rinsed with DI, destained with acidic ethanol (70% ethanol in diluted hydrochloric acid), counterstained with eosin solution (eosin phloxine) and then dehydrated with 95% ethanol, 100% ethanol and xylene sequentially. Slides were then covered using a per−mounting solution. The stained tissue sections were investigated under a digital light microscope (Nikon Instrument Inc., Minato, Tokyo, Japan) at a magnification of ×40 by an expert clinical pathologist, Dr. Sarawuth Kongkarnka, MD., at the Department of Pathology, Faculty of Medicine, Chiang Mai University.
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