Luria broth

Luria broth, Tris, HEPES and PIPES were obtained from Carl Roth GmbH (Karlsruhe, Germany). Ni-NTA beads were purchased from Qiagen (Hilden, Germany) and Biobeads were ordered from Bio-Rad Laboratories GmbH (München, Germany). ATP, ADP, ADP-βS, sodium meta-arsenite, phosphoenol pyruvate, lithium chloride and EDTA were purchased from Sigma-Aldrich GmbH (Schnelldorf, Germany). Complete protease inhibitor cocktail tablets, pyruvate kinase and lactate dehydrogenase were bought from Roche Diagnostics Deutschland GmbH (Mannheim, Germany) and Run Blue precast SDS–polyacrylamide gel electrophoresis gels from Expedeon Ltd (Cambridge, UK). Lipids were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). All other compounds were from AppliChem GmbH (Darmstadt, Germany). For size-exclusion chromatography (SEC), Sephadex 200 10/300GL and PD10 column for buffer exchange were from GE Healthcare Europe GmbH (Freiburg, Germany). Primers for mutations were ordered from Eurofins Genomics GmbH (Ebersberg, Germany).

Bacterial strains and plasmids used in the present study are shown in Table 1. E. coli were grown with agitation at 37°C in Luria broth (LB; Roth) or LB Agar, while gram-positive bacteria (S. aureus, E. faecalis 12030 and E. faecium) were grown in Tryptic Soy Broth (TSB) or Tryptic Soy Agar (TSA) at 37°C without agitation. Antibiotics (all purchased from Sigma) were added as indicated.