Hk320

Plasma sCD14 concentrations were analysed using an ELISA (HK320, HycultBiotech) following the manufacturer’s instructions. The detection range of this assay was specified to be 1.56 to 100 ng/ml.

Determinations of total cholesterol, triglyceride, HDL cholesterol, apoA-I and apoB concentrations were carried out by using Abbott Architect reagents (Abbott Laboratories, Abbott Park, IL, USA) in an accredited clinical laboratory, Forensic Toxicology Unit at the National Institute for Health and Welfare, Helsinki, Finland. Low density lipoprotein (LDL) cholesterol was calculated by Friedewald formula. For standardizing measurements, the laboratory has taken part in Lipid Standardization Program organized by CDC, Atlanta, USA. During the course of the laboratory measurements of the study samples in 2016, the precision was characterized by the coefficient of variation (mean ± SD) and the accuracy by systematic error (bias) (mean ± SD). Precision and accuracy were 0.6% ± 0.1 and 0.7% ± 0.5 for total cholesterol, 2.0% ± 0.4 and -2.3% ± 2.0 for triglycerides, 1.3% ± 0.3 and 3.0% ± 1.9 for HDL cholesterol, 1.3% ± 0.6 and 2.9% ± 1.5 for apoA-I and 1.7% ± 0.9 and -3.1% ± 1.7 for apoB, respectively.
Phospholipid transfer activity by PLTP was measured using a lipoprotein-independent assay as previously described [33 (link)]. LBP and sCD14 concentrations were determined by use of commercial assays from Hycult Biotech (Hycult Biotech Inc, Wayne, PA, USA), catalog numbers HK315-01 and HK320, respectively, according to the manufacturer’s suggestions.