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Oligonucleotides and gene blocks

Manufactured by Integrated DNA Technologies
Sourced in United States

Oligonucleotides and gene blocks are synthetic DNA molecules used in various molecular biology applications. They serve as building blocks for DNA synthesis and manipulation. Oligonucleotides are short, single-stranded DNA sequences, while gene blocks are longer, double-stranded DNA fragments. These products are designed to meet the specific needs of researchers and provide a reliable source of customized genetic materials.

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3 protocols using oligonucleotides and gene blocks

1

Reagent Sourcing for Viral Studies

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All chemicals were of analytical grade and were purchased from Sigma–Aldrich (St. Louis, MO, USA) unless otherwise indicated. All enzymes and related buffers were purchased from New England Biolabs (NEB, Ipswich, MA, USA) unless otherwise indicated. All oligonucleotides and gene blocks (summarized in Supplementary Tables S1–S4) were obtained from Integrated DNA Technologies (IDT, Coralville, IA, USA). Virus genomic RNA and inactivated virions (Supplementary Table S5) were obtained from BEI Resources (Manassas, VA, USA).
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2

Comprehensive Molecular Biology Protocol

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All chemicals were of analytical grade and were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.) unless otherwise indicated. Bacterial growth media were purchased from Thermo Fisher Scientific (Waltham, MA). Bacterial strains and all pure enzymes and related buffers were purchased from New England Biolabs (NEB, Ipswich, MA) unless otherwise indicated. KlenTaq1 was purchased from DNA Polymerase Technologies (St. Louis, MO). All oligonucleotides and gene blocks were obtained from Integrated DNA Technologies (IDT, Coralville, IA, U.S.A.). Oligonucleotide and gene block sequences are summarized in S1 Table.
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3

Analytical Grade Reagents Protocol

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All chemicals were of analytical grade and were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.) unless otherwise indicated. All enzymes and related buffers were purchased from New England Biolabs (NEB, Ipswich, MA) unless otherwise indicated. All oligonucleotides and gene blocks (summarized in S1 Table) were obtained from Integrated DNA Technologies (IDT, Coralville, IA, U.S.A.).
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