Ec plan apochromat 63 1.40 oil dic m27 objective

All microscopy images were acquired via Zen software on a Zeiss 700 LSM mounted on an Axio Observer.Z1 using a Plan-Apochromat 20×/0.8 M27 or an EC Plan-Apochromat 63×/1.40 Oil DIC M27 objective (Carl Zeiss Microscopy, Thornwood, NY) or via LAS AF SPE Core software on a Leica TCS SPE mounted on a Leica DM2500 using an ACS APO 20×/0.60 IMM CORR-/D or an ACS APO 63×/1.30 Oil CS 0.17/E objective (Leica Microsystems). Maximum projections, image rotation, and cropping were performed in ImageJ (National Institutes of Health, Bethesda, MD; Abramoff et al., 2004 ). Figure panels were assembled in Illustrator (Adobe, San Jose, CA). To aid in visualization, Figures 1,B–C′ and E–G′; 3, A–F; 5, B–G; 7, A–B′′ and D–D′′; and 9, A–C′′′; and Supplemental Movie S1, A–D, were brightened by 30% using Photoshop (Adobe).