Alexa 546 conjugated donkey anti mouse igg

Manufactured by Thermo Fisher Scientific

Alexa Fluor 546-conjugated donkey anti-mouse IgG is a secondary antibody that binds to mouse immunoglobulin G (IgG) and is labeled with the Alexa Fluor 546 fluorescent dye. This antibody can be used for detection and visualization of mouse primary antibodies in various immunoassay techniques, such as immunofluorescence, flow cytometry, and western blotting.

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Lab products found in correlation

Lsm 710, by Zeiss (2 mentions) Ab14106, by Abcam (1 mentions) Alexa 647 conjugated donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa 647 conjugated donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Four well chamber slide, by Thermo Fisher Scientific (1 mentions) Alexa 488 conjugated donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Dapi fluoromount g, by Southern Biotech (1 mentions)

Spelling variants of this product

Alexa 546 (139 citations) Alexa Fluor 546-conjugated donkey anti-mouse IgG (9 citations) Donkey anti-mouse Alexa 546 (5 citations) Mouse anti-IgG (2 citations) Alexa Fluor 546-conjugated donkey anti-mouse IgG (h4l) (2 citations) Alexa Fluor 546-conjugated donkey anti-mouse IgG antibody (2 citations) Donkey anti-mouse IgG conjugated to Alexa Fluor 546 (2 citations)

2 protocols using alexa 546 conjugated donkey anti mouse igg

Immunohistochemical Analysis of Zebrafish

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Antibody staining was executed as previously described^{20 (link)} using either zebrafish embryos or sections. The primary antibodies, sources and dilutions used were anti-Col2A1 (II-II6B3; DSHB; 1:100), anti-eGFP (TP401; Torrey Pines; 1:500), zns-5 (AB_10013796; ZIRC; 1:200) and anti-SM22 alpha/Transgelin (ab14106; Abcam; 1:250). Secondary antibodies were all from Invitrogen and used at 1:500: Alexa 488-conjugated donkey anti-rabbit immunoglobulin G (IgG; catalogue number A-21206), Alexa 546-conjugated donkey anti-mouse IgG (catalogue number A10036), Alexa 647-conjugated donkey anti-rabbit IgG (catalogue number A-31573) and Alexa 647-conjugated donkey anti-mouse IgG (catalogue number A-31571).

Tzung K.W., Lalonde R.L., Prummel K.D., Mahabaleshwar H., Moran H.R., Stundl J., Cass A.N., Le Y., Lea R., Dorey K., Tomecka M.J., Zhang C., Brombacher E.C., White W.T., Roehl H.H., Tulenko F.J., Winkler C., Currie P.D., Amaya E., Davis M.C., Bronner M.E., Mosimann C, & Carney T.J. (2023). A median fin derived from the lateral plate mesoderm and the origin of paired fins. Nature, 618(7965), 543-549.

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Immunofluorescence Imaging of CRC Cells

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Pretreated CRC cells were plated in four-well chamber slides (Nunc, Denmark) 24h before experiment. The cells were probed with corresponding antibodies for 1h at RT and incubated with Alexa 546-conjugated donkey anti-mouse IgG, followed by Alexa-488-conjugated donkey anti-rabbit IgG (Invitrogen). Afterwards the slides were mounted in DAPI Fluoromount-G (SouthernBiotech, Birmingham, USA) and images were captured using a laser-scanning confocal microscope (LSM-710, Zeiss, Germany).

Zhao H.J., Ren L.L., Wang Z.H., Sun T.T., Yu Y.N., Wang Y.C., Yan T.T., Zou W., He J., Zhang Y., Hong J, & Fang J.Y. (2014). MiR-194 Deregulation Contributes To Colorectal Carcinogenesis via Targeting AKT2 Pathway. Theranostics, 4(12), 1193-1208.

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