Cystoisospora suis oocysts (strain Wien 1) were obtain from experimental infected suckling piglets. Piglets were raised with the sow in the animal facilities of the Institute of Parasitology, University of Veterinary Medicine Vienna, Austria. Infection of piglets, oocyst collection, oocyst isolation, oocyst purification and excystation were performed as described previously [7 (link)]. In deviation to the original protocol, after NaOCl treatment and washing, the oocysts were vortexed three times for 45 s with Precellys® glass beads (Peqlab, Erlangen, Germany) in 2% sodium taurocholate hydrate (Sigma-Aldrich, St. Louis, USA) in DMEM/Ham’s F-12 culture medium (Gibco, Thermo Fisher Scientific, Waltham, USA).
Precellys glass beads
Manufactured by Avantor
Sourced in Germany
Precellys® glass beads are a type of laboratory equipment used for sample preparation. They are made of high-quality glass and are designed for efficient homogenization and disruption of a variety of sample types, including tissues, cells, and microorganisms. The core function of Precellys® glass beads is to provide a mechanical means of disrupting samples to release their contents for further analysis or processing.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using precellys glass beads
1
Isolation and Purification of Cystoisospora suis Oocysts
2
Cystoisospora suis Oocyst Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cystoisospora suis oocysts (strain Wien 1) were obtain from experimental infected suckling piglets. Piglets were raised with the sow in the animal facilities of the Institute of Parasitology, University of Veterinary Medicine Vienna, Austria. Infection of piglets, oocyst collection, oocyst isolation, oocyst purification and excystation were performed as described previously [7] . In deviation to the original protocol, after NaOCl treatment and washing, the oocysts were vortexed three times for 45 sec with Precellys® glass beads (Peqlab, Erlangen, Germany) in 2% sodium taurocholate hydrate (Sigma-Aldrich, St. Louis, USA) in DMEM/Ham's F-12 culture medium (Gibco, Thermo Fisher Scientific, Waltham, USA).
3
Cystoisospora suis Oocyst Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cystoisospora suis oocysts (strain Wien 1) were obtain from experimental infected suckling piglets. Piglets were raised with the sow in the animal facilities of the Institute of Parasitology, University of Veterinary Medicine Vienna, Austria. Infection of piglets, oocyst collection, oocyst isolation, oocyst purification and excystation were performed as described previously [7] (link). In deviation to the original protocol, after NaOCl treatment and washing, the oocysts were vortexed three times for 45 sec with Precellys® glass beads (Peqlab, Erlangen, Germany) in 2% sodium taurocholate hydrate (Sigma-Aldrich, St. Louis, USA) in DMEM/Ham's F-12 culture medium (Gibco, Thermo Fisher Scientific, Waltham, USA).
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