Ab28466

Manufactured by Abcam

Ab28466 is a laboratory reagent product from Abcam. It is a monoclonal antibody that can be used for research purposes. The core function of this product is to serve as a detection tool in various laboratory techniques, but no further details on its intended use can be provided in an unbiased manner.

Automatically generated - may contain errors

Lab products found in correlation

Ab28364, by Abcam (1 mentions) Ab51745, by Abcam (1 mentions) Ab1791, by Abcam (1 mentions) Ab40854, by Abcam (1 mentions) Nb100 479, by Novus Biologicals (1 mentions)

2 protocols using ab28466

Immunohistochemical Analysis of ONECUT2 in Ovarian Cancer

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Immunohistochemistry was carried out as previously described.19, 20 The tumor tissue samples of ovarian cancer patients were provided by The Third Affiliated Hospital of Sun Yat‐sen University (Guangzhou, China). For human ovarian cancer tissue, the primary antibody was rabbit anti‐human ONECUT2 polyclonal antibody (1:500 dilution, ab28466; Abcam). For tumor tissue of the mouse, the primary antibody was rabbit anti‐mouse CD31 polyclonal antibodies (1:50 dilution, ab28364; Abcam), rabbit anti‐mouse VEGFA polyclonal antibody (1:100 dilution, ab51745; Abcam), rabbit anti‐mouse HIF‐1α monoclonal antibody (1:200 dilution, 36169; Cell Signaling Technology), and rabbit anti‐mouse ONECUT2 polyclonal antibody (1:500 dilution, ab28466; Abcam). The biotinylated HRP‐conjugated secondary antibody was a goat anti‐rabbit IgG.

Lu T., Wu B., Yu Y., Zhu W., Zhang S., Zhang Y., Guo J, & Deng N. (2018). Blockade of ONECUT2 expression in ovarian cancer inhibited tumor cell proliferation, migration, invasion and angiogenesis. Cancer Science, 109(7), 2221-2234.

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Western Blot for Key Protein Targets

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Western blotting was performed as described previously^{50 (link)}. The blots were first incubated with anti-ONECUT2 (21916-1-AP, Proteintech; ab28466, Abcam), anti-SMAD3 (ab40854, Abcam), anti-HIF1α (NB100-479, Novus), anti-H3 (ab1791, Abcam) or anti-GAPDH (ab1791, Abcam), and then with 1:10000 anti-rabbit secondary antibody (7074, Cell Signaling).

Guo H., Ci X., Ahmed M., Hua J.T., Soares F., Lin D., Puca L., Vosoughi A., Xue H., Li E., Su P., Chen S., Nguyen T., Liang Y., Zhang Y., Xu X., Xu J., Sheahan A.V., Ba-Alawi W., Zhang S., Mahamud O., Vellanki R.N., Gleave M., Bristow R.G., Haibe-Kains B., Poirier J.T., Rudin C.M., Tsao M.S., Wouters B.G., Fazli L., Feng F.Y., Ellis L., van der Kwast T., Berlin A., Koritzinsky M., Boutros P.C., Zoubeidi A., Beltran H., Wang Y, & He H.H. (2019). ONECUT2 is a driver of neuroendocrine prostate cancer. Nature Communications, 10, 278.

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