Deuteriumoxide (D2O), N,N,N′,N′-tetramethylethylenediamine (TEMED), ammonium persulphate (APS), phosphotungstic acid hydrate (H3[PW12O40]·xH2O), glycine, disodium phosphate (Na2HPO4), sodiu, dodecyl sulfate (SDS), horse heart cytochrome c (Cyt c) and bromophenol blue were bought from Sigma-Aldrich. Zirconium oxychloride octahydrate and diethyl ether were purchased from ChemLab. Aqueous hydrochloric acid (37%), potassium hydrogen carbonate and ammonium ceriumIV nitrate (CAN) were obtained from Acros organics. Ethanol, sodium hydrogen carbonate, aqueous ortho-phosphoric acid (85%), diethyalaminehydrochloride and protein ladders were acquired from Thermo Fisher Scientific. MEthanol and monosodiumphosphate (NaH2PO4) were purchased from VWR. Potasium chloride, tris(hydroxymethyl)aminomethane (TRIS) and acrylamide:bisacrylamide (29:1) solution (30%) were procured from Applichem. [Ce(α-PW11O39)2]10− (CeK ) was synthesized according to (Griffith et al., 2000 (link)) [Zr(α-PW11O39)2]10− (ZrK ) was synthesized following a slightly altered procedure from (Sokolov et al., 2007 (link)).
Disodium phosphate na2hpo4
Manufactured by Merck Group
Sourced in United States
Disodium phosphate (Na2HPO4) is a chemical compound that is commonly used as a buffer, pH adjuster, and stabilizer in various laboratory applications. It is a white, crystalline powder that is highly soluble in water. Disodium phosphate is a versatile compound that can be used in a variety of scientific and industrial processes.
Automatically generated - may contain errors
Lab products found in correlation
Monosodium phosphate nah2po4, by Merck Group (3 mentions)
Ethanol, by Thermo Fisher Scientific (2 mentions)
Acrylamide bisacrylamide, by AppliChem (2 mentions)
Bromophenol blue, by Merck Group (1 mentions)
Sodium hydrogen carbonate, by Thermo Fisher Scientific (1 mentions)
Ammonium persulphate, by Merck Group (1 mentions)
Horse heart cytochrome c cyt c, by Merck Group (1 mentions)
Monosodiumphosphate nah2po4, by Avantor (1 mentions)
Tris hydroxymethyl aminomethane tris, by AppliChem (1 mentions)
Diethyl ether, by Chem-Lab (1 mentions)
Triton x 100, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
Tris base, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Horseradish peroxidase, by Merck Group (1 mentions)
Glucose oxidase, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by HiMedia (1 mentions)
Calcium chloride, by HiMedia (1 mentions)
8 protocols using disodium phosphate na2hpo4
1
Polyoxometalate-Based Protein Electrophoresis
2
Immunohistochemical Analysis of Oral Cancer
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FcMeOH (>95%) was purchased
from Tokyo Chemical Industry, Japan. Monopotassium phosphate (KH2PO4, 99.5%) and sodium chloride (NaCl, 99.5%) were
purchased from SHOWA, Japan. Disodium phosphate (Na2HPO4, 99%), monosodium phosphate (NaH2PO4, 99%), hydrogen peroxide (H2O2, pure, 30%
w/w), and Tris base were purchased from Sigma-Aldrich. Anti-IL-6 antibody
and goat anti-mouse IgG were bought from Abcam, UK. Bovine serum albumin
(BSA) was purchased from Sigma-Aldrich. Xylene and Triton X-100 were
purchased from Fisher. Ethanol (99.5%) was purchased from ECHO. Tween
20 was bought from Merck, Germany. Human oral cancer tissue array
(product number T271b), oral cancer tissue array (product number T273),
and human oral cancer tissue slices (product numbers HuCAT481 and
HuCAT503) were purchased from Biomax. Details of human oral cancer
samples are presented in the Supporting Information (SI)Table S1 , including the patients’ age
and sex of the pathology sites. Deionized water was obtained from
Sartorius (18.2 MΩ·cm). The conductive silver paint was
bought from Pelco. Polyimide was purchased from Kapton (DuPont). Parafilm
was purchased from Parafilm M (Merck, Germany). Gold wire (25 μm)
was purchased from Tanaka Electronics, Singapore. Sylgard 184 A&B
was bought from Uni-Onward, Taiwan.
from Tokyo Chemical Industry, Japan. Monopotassium phosphate (KH2PO4, 99.5%) and sodium chloride (NaCl, 99.5%) were
purchased from SHOWA, Japan. Disodium phosphate (Na2HPO4, 99%), monosodium phosphate (NaH2PO4, 99%), hydrogen peroxide (H2O2, pure, 30%
w/w), and Tris base were purchased from Sigma-Aldrich. Anti-IL-6 antibody
and goat anti-mouse IgG were bought from Abcam, UK. Bovine serum albumin
(BSA) was purchased from Sigma-Aldrich. Xylene and Triton X-100 were
purchased from Fisher. Ethanol (99.5%) was purchased from ECHO. Tween
20 was bought from Merck, Germany. Human oral cancer tissue array
(product number T271b), oral cancer tissue array (product number T273),
and human oral cancer tissue slices (product numbers HuCAT481 and
HuCAT503) were purchased from Biomax. Details of human oral cancer
samples are presented in the Supporting Information (SI)
and sex of the pathology sites. Deionized water was obtained from
Sartorius (18.2 MΩ·cm). The conductive silver paint was
bought from Pelco. Polyimide was purchased from Kapton (DuPont). Parafilm
was purchased from Parafilm M (Merck, Germany). Gold wire (25 μm)
was purchased from Tanaka Electronics, Singapore. Sylgard 184 A&B
was bought from Uni-Onward, Taiwan.
3
Insulin Enzyme-Linked Immunosorbent Assay
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Dulbecco’s modified Eagle’s
medium (DMEM), fetal bovine serum (FBS), Penstrep, and trypsin-EDTA
were obtained from Gibco. Monosodium phosphate (NaH2PO4), Rat/Mouse
(EZRMI-13K) Insulin ELISA Assay Kit, and ethanol were purchased from
Merck. Magnesium chloride (MgCl2), glucose, calcium chloride
(CaCl2), potassium chloride (KCl), HEPES, monopotassium
phosphate (KH2PO4), sodium chloride (NaCl),
dipotassium phosphate (K2HPO4), sodium bicarbonate
(NaHCO3), formaldehyde, 2-propanol, BSA, and Bradford were
purchased from Himedia, India. Glucose oxidase, 4-aminoantipyrine N-ethyl-N-sulfopropyl-M-toluidine, horseradish peroxidase, disodium phosphate (Na2HPO4), and chloroform were purchased from Sigma, India.
Trizol was obtained from Ambion Life Technology.
medium (DMEM), fetal bovine serum (FBS), Penstrep, and trypsin-EDTA
were obtained from Gibco. Monosodium phosphate (NaH2PO4), Rat/Mouse
(EZRMI-13K) Insulin ELISA Assay Kit, and ethanol were purchased from
Merck. Magnesium chloride (MgCl2), glucose, calcium chloride
(CaCl2), potassium chloride (KCl), HEPES, monopotassium
phosphate (KH2PO4), sodium chloride (NaCl),
dipotassium phosphate (K2HPO4), sodium bicarbonate
(NaHCO3), formaldehyde, 2-propanol, BSA, and Bradford were
purchased from Himedia, India. Glucose oxidase, 4-aminoantipyrine N-ethyl-N-sulfopropyl-M-toluidine, horseradish peroxidase, disodium phosphate (Na2HPO4), and chloroform were purchased from Sigma, India.
Trizol was obtained from Ambion Life Technology.
4
Polyoxometalate-Protein Interaction Assay
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Albumin from chicken egg white lyophilized powder (ovalbumin, OVA), deuteriumoxide (D2O), N,N,N′,N′-tetramethylethylenediamine (TEMED), and disodium phosphate (Na2HPO4) were bought from Sigma-Aldrich. Hafnium oxychloride octahydrate (HfOCl2·8H2O) and diethyl ether (Et2O) were purchased from ChemLab. Aqueous hydrochloric acid (HCl, 37%), acetic acid (CH3COOH), sodium acetate (CH3COONa), and potassium hydrogen carbonate (NaHCO3) were obtained from Acros Organics. MEthanol and monosodiumphosphate (NaH2PO4) were purchased from VWR. Ethanol, aqueous orthophosphoric acid (H3PO4, 85%), and protein ladders were acquired from Thermo Fisher Scientific. Potassium chloride (KCl), tris(hydroxymethyl)aminomethane (TRIS), and acrylamide:bisacrylamide (29:1) solution (30%) were procured from AppliChem. All compounds were purchased in the highest available purity and were used without further purification. α-/β-K6P2W18O62·14/19H2O (Contant et al., 1990 ), α2-K10P2W17O61·20H2O (Contant et al., 1990 ), and K16[Hf(α2-P2W17O61)2]·19H2O (Kato et al., 2006 (link)) were synthesized according to the reported literature procedures.
5
Chitosan-based Hydrogel Synthesis
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Sodium carbonate (Na2CO3), lithium bromide (LiBr), acetic acid (CH3COOH), chitosan (DD = 78%; viscosity average molecular weight = 0.59 × 106 g/mol), (N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide, calcium chloride dihydrate (CaCl2 × 2H2O), phosphate buffered saline (purchased in a tablet form with one tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C), ethanol (C2H5OH) and di-sodium phosphate (Na2HPO4) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
6
Layer-by-Layer Assembly of Polyelectrolytes
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Sodium chloride (NaCl), sodium hydroxide
standard solution (0.1 M, NaOH), poly(acrylic acid) (PAA, Mw ∼ 1.2 kDa), branched polyethylenimine
(BPEI, Mw = 750 kDa, Mn = 60 kDa), poly(methacrylic acid) (PMAA, Mw = 60 kDa), poly(4-vinylpyridine) (P4VP, Mw = 160 kDa) glutaraldehyde, sodium acetate buffer (NaOAc),
sodium dihydrogen phosphate (NaH2PO4), and disodium
phosphate (Na2HPO4) were obtained from Sigma-Aldrich.
Hydrochloric acid (1 N standard solution, HCl) was purchased from
Acros Organics. Hydrogen peroxide (30% in water, H2O2) was obtained from Fisher Scientific Inc. Cross-linkable
quaternized poly(4-vinylpyridine) (qP4VP, 160 kDa) was synthesized,
as described in section 2.3. All chemicals were used without further
purification steps. All solutions were prepared using ultrapure water
(Milli-Q, <18.2 MΩ cm). For LbL deposition, qP4VP and PAA
solutions were prepared at a concentration of 0.2 mg/mL in 10 mM sodium
phosphate buffer and adjusted to pH 6.5. The salinity solutions were
prepared by adding Sodium chloride powder to the phosphate buffer
solutions at pH 8.1.
standard solution (0.1 M, NaOH), poly(acrylic acid) (PAA, Mw ∼ 1.2 kDa), branched polyethylenimine
(BPEI, Mw = 750 kDa, Mn = 60 kDa), poly(methacrylic acid) (PMAA, Mw = 60 kDa), poly(4-vinylpyridine) (P4VP, Mw = 160 kDa) glutaraldehyde, sodium acetate buffer (NaOAc),
sodium dihydrogen phosphate (NaH2PO4), and disodium
phosphate (Na2HPO4) were obtained from Sigma-Aldrich.
Hydrochloric acid (1 N standard solution, HCl) was purchased from
Acros Organics. Hydrogen peroxide (30% in water, H2O2) was obtained from Fisher Scientific Inc. Cross-linkable
quaternized poly(4-vinylpyridine) (qP4VP, 160 kDa) was synthesized,
as described in section 2.3. All chemicals were used without further
purification steps. All solutions were prepared using ultrapure water
(Milli-Q, <18.2 MΩ cm). For LbL deposition, qP4VP and PAA
solutions were prepared at a concentration of 0.2 mg/mL in 10 mM sodium
phosphate buffer and adjusted to pH 6.5. The salinity solutions were
prepared by adding Sodium chloride powder to the phosphate buffer
solutions at pH 8.1.
7
Hyaluronic Acid Hydrogel for Drug Delivery
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Poly(ethylene glycol) methyl ether methacrylate (OEGMA, average Mw = 300 g/mol), di(ethylene glycol) methyl ether methacrylate (MEO2MA), hyaluronic acid methacrylate (MeHA, degree of substitution: 20%-50%, MW 120,000-150,000), di(ethylene glycol) diacrylate (DEGDA 75.00%), hyaluronidase from bovine testes (Type I-S, 400-1000 units/mg, solid), potassium persulfate (KPS, 99,99%), sodium dodecyl sulfate (SDS), sodium hydroxide (NaOH), sodium chloride (NaCl), potassium chloride (KCl), monosodium phosphate (NaH2PO4) and disodium phosphate (Na2HPO4), were purchased from Sigma Aldrich (Saint Louis, Missouri, MO, USA). Doxorubicin hydrochloride (DOX) was purchased from LC Laboratories (Woburn, MA, USA). Mitoxantrone dihydrochloride (MTX) was purchased from MCE (NJ, USA). For the preparation of all solutions, deionized water (Hydrolab/HLP, Straszyn, Poland) with conductivity 0.055 μS·cm−1 was used.
8
Analytical LC-MS Reagent Preparation
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LC-MS grade acetonitrile (ACN), methanol (MeOH) and 2-butanol (2-BuOH; ≥ 99.5%), disodium phosphate (Na2HPO4) and citric acid were purchased from Sigma Aldrich (Isle d'Abeau, France).
Formic acid (FA) (LC-MS grade) and ammonium acetate (AA) (analytical reagent grade) were obtained from Fisher scientific (Waltham, MA, USA). Sodium bicarbonate was from Laurylab (Brindas, France) and hydrochloric acid from Carlo Erba Reagents (Val-de-Reuil, France). Water was purified by an Elga purification system (Veolia, Paris, France). Mobile phases (all proportion expressed as v/v) were filtered through 0.2 µm PTFE membranes (Merck, Germany) before use.
Formic acid (FA) (LC-MS grade) and ammonium acetate (AA) (analytical reagent grade) were obtained from Fisher scientific (Waltham, MA, USA). Sodium bicarbonate was from Laurylab (Brindas, France) and hydrochloric acid from Carlo Erba Reagents (Val-de-Reuil, France). Water was purified by an Elga purification system (Veolia, Paris, France). Mobile phases (all proportion expressed as v/v) were filtered through 0.2 µm PTFE membranes (Merck, Germany) before use.
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