Anti beta actin ab8227

Manufactured by Abcam

Sourced in United States

Anti-beta-actin (ab8227) is a rabbit polyclonal antibody that recognizes the beta-actin protein. It is designed for use in various applications such as Western blotting, immunohistochemistry, and immunocytochemistry to detect the expression of beta-actin.

Automatically generated - may contain errors

Lab products found in correlation

Anti zeb1, by Cell Signaling Technology (1 mentions) Anti mdm2 sc 813, by Santa Cruz Biotechnology (1 mentions) Laminin l2020, by Merck Group (1 mentions) Anti nestin sc 23927, by Santa Cruz Biotechnology (1 mentions) Anti p53 sc6243, by Santa Cruz Biotechnology (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions)

3 protocols using anti beta actin ab8227

Antibody Validation and Cellular Markers

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AMG232 and RG7112 were purchased from APExBIO Technology. Anti-p21 (#2947), anti-PARP (#9542), and anti-ZEB1 (#3396) were purchased from Cell Signaling, anti-p53 (sc-6243), anti-MDM2 (sc-813), and anti-Nestin (sc-23927) were purchased from Santa Cruz Biotechnology and anti-beta-actin (ab8227) was purchased from Abcam. Alexa Fluor 488 anti-rabbit secondary antibody (A11008) was purchased from Thermo Fisher Scientific. Laminin (L2020) was purchased from Sigma and Hoechest33342 (H3570) was purchased from Life Technologies.

Her N.G., Oh J.W., Oh Y.J., Han S., Cho H.J., Lee Y., Ryu G.H, & Nam D.H. (2018). Potent effect of the MDM2 inhibitor AMG232 on suppression of glioblastoma stem cells. Cell Death & Disease, 9(8), 792.

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Western Blot Antibody Validation

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Western blotting was carried out according to standard methods. We used the following antibodies; anti-AhR (H-211), anti-GAPDH (FL-335) and anti-PDC-E2 (C-1) antibodies (Santa Cruz Biotechnology), anti-beta-actin (ab8227) and anti-PDC-E2 (ab110332) antibodies (Abcam), anti-GAPDH antibody (MA1–22670, Thermo, MA, USA), anti-cytochrome c antibody (556433,BD Pharmingen, NJ, USA), anti-histone H3 (#9715), anti-FLAG (#2368) and anti-PKM2 (#4053) antibodies (Cell Signaling Technology). Polyclonal anti-PKM2 antibody against a PKM2-specific peptide (LRRLAPITSDPTEATAVGAV) was raised in rabbits.

Matsuda S., Adachi J., Ihara M., Tanuma N., Shima H., Kakizuka A., Ikura M., Ikura T, & Matsuda T. (2015). Nuclear pyruvate kinase M2 complex serves as a transcriptional coactivator of arylhydrocarbon receptor. Nucleic Acids Research, 44(2), 636-647.

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Western Blot Analysis of JMJD5 Protein

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All the proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Millipore, MA, USA). The membrane was treated with 5% skim milk in TBST buffer, anti-JMJD5 antibody (Cat. No., ab36104, 1:3000 dilutions), and anti-beta-actin (ab8227) from Abcam Inc., (Cambridge, MA, USA) overnight at 4°C. Subsequently, the membrane was incubated with horseradish peroxidase-conjugated secondary antibody (Cat. No., ab6721, Abcam Inc., Cambridge, MA, USA).

Wang Y., Liu L., Ji F., Jiang J., Yu Y., Sheng S, & Li H. (2018). Soybean (Glycine max) prevents the progression of breast cancer cells by downregulating the level of histone demethylase JMJD5. Journal of cancer research and therapeutics, 14(Supplement).

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