Mice were anesthetized by i.p. injection of avertin (2,2,2-tribromoethanol, Sigma–Aldrich) in 2% solution at a dose of 400 mg kg-1 bodyweight and placed on a controlled heating pad (Föhr Medical Instruments, Seeheim-Ober Beerbach, Germany) in supine position. Additional doses of avertin (each 10% of the initial dose) were applied during experiments if appropriate to maintain depth of anesthesia. A miniature pressure-volume catheter (model SPR-839, Millar Instruments, Houston, TX, United States) was inserted via the right carotid artery and placed in the left ventricle. Increasing doses of dobutamine were administered into the cannulated left external jugular vein using an automated syringe pump (B. Braun, Melsungen, Germany). Data were recorded using the MPVS-400 system (Millar Instruments) and Chart5 software (ADInstruments, Bella Vista, NSW, Australia). At the end of experiments, animals were euthanized by avertin overdose, and hearts were excised, weighed, and stored at -80°C until further examination. Hemodynamic data were analyzed using Chart5 software (ADInstruments) and PVAN software (Millar Instruments).
Chart 5
Manufactured by ADInstruments
Sourced in Australia, United States, United Kingdom, Germany
Chart 5 is a data acquisition and analysis software suite designed for scientific research and experimentation. It provides a comprehensive platform for real-time data recording, visualization, and analysis. The software supports a wide range of laboratory instruments and sensors, enabling users to capture, display, and interpret experimental data.
Automatically generated - may contain errors
Lab products found in correlation
Powerlab, by ADInstruments (10 mentions)
Oxylet, by Harvard Apparatus (10 mentions)
Powerlab system, by ADInstruments (3 mentions)
Spr 839, by Millar (2 mentions)
Pvan software, by Millar (2 mentions)
Powerlab 8 30, by ADInstruments (2 mentions)
Powerlab device, by ADInstruments (2 mentions)
Spr 1000, by Millar (2 mentions)
1.4 f pressure catheter, by Millar (2 mentions)
10 mhz probe, by GE Healthcare (2 mentions)
Ml119 powerlab interface, by ADInstruments (2 mentions)
Scope 4, by ADInstruments (2 mentions)
Bio amp, by ADInstruments (2 mentions)
Aria 1 pv conductance system, by ADInstruments (2 mentions)
Powerlab 8, by ADInstruments (2 mentions)
Avertin, by Merck Group (1 mentions)
Automated syringe pump, by B. Braun (1 mentions)
Mpvs 400 system, by Millar (1 mentions)
Coda system, by Kent Scientific (1 mentions)
Electromyogram acquisition system, by ADInstruments (1 mentions)
77 protocols using chart 5
1
Hemodynamic Assessment in Anesthetized Mice
2
Measuring Blood Pressure and Urinary Protein in Pregnant Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The systolic blood pressure was noninvasively measured by a volume pressure recording sensor and an occlusion tail‐cuff (CODA System, Kent Scientific). This method shows good agreement with radiotelemetry measurements of blood pressure.47 Blood pressure was measured at the same time daily (±1 hour) while the mice were kept warm using a warming pad. Twenty‐four‐hour urine was collected for analysis using metabolic cages (Nalgene). Mice were trained in metabolic cages for 2 days prior to urine collection. On GD18.5, the mean arterial pressure was determined by cannulating the right carotid artery with a mouse jugular catheter connected to a pressure transducer and an amplifier unit, just prior to euthanasia. The amplifier was connected to a data acquisition module and mean arterial pressure was recorded on a personal computer by Chart 5 Software (AD Instruments, Inc). All of the mice were euthanized on GD18.5 before delivery when their serum and organs were collected. We quantified urinary albumin by ELISA (Exocell) and measured urinary creatinine by a picric acid colorimetric assay (Exocell). We used the ratio of urinary albumin to urinary creatinine as an index of urinary protein.
3
EEG Analysis of Alcohol Withdrawal
4
Cardiac Function Measurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cardiac function indices, including SAP, LVSP, +dp/dt, -dp/dt, HR and CSF, were checked before treatment and 30/60 min post-treatment, using the ALCB10-MPA Cardiac Function Analysis System (Alcott Biotech), according to the manufacturer's instructions. Post-treatment hemodynamic parameters were measured by catheterization (SRP-320/PVAN 3.2, Millar Instruments, Inc., Houston, TX, USA; Chart 5 software, ADInstruments, Dunedin, New Zealand), as previously described (24 (link)).
5
Mitochondrial Accumulation Ratio Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RLM (0.5 mg protein/ml) were incubated in 4 ml KCl buffer supplemented with rotenone (4 µg/ml) and TPMP (2.5 µM) and the respective MitoC variant (2.5 µM) in open plastic tubes (Sarsted®) in a shaking water bath at 37 °C. Respiration was initiated by the addition of succinate (4 mM). When indicated FCCP (1 µM) was added 4 min later. Five min later mitochondria were pelleted by centrifugation (7500 x g for 5 min at 4 °C). Supernatants were removed and stored at 4 °C in eppendorf tubes until analysis. Mitochondrial pellets were extracted in 250 µL buffer B followed by centrifugation (7500 x g for 5 min). The supernatant (250 µL) was removed and stored at 4 °C in glass vials (Chromacol®). All samples were diluted to 25% ACN with buffer A, and separated by RP-HPLC as described above. TPMP and MitoC standards were used to determine peak areas and elution times, using the Chart 5 software (ADInstruments®). Accumulation ratios (ACR) between the mitochondrial matrix and the extra-mitochondrial environment were calculated by normalizing the peak areas to mitochondrial volume (0.5 µL/mg protein [28] ) and to 4 ml for the extra-mitochondrial environment.
6
Visceromotor Responses to Colonic Distension
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three days before CRD, 2 electrodes were implanted in the abdominal external oblique musculature of mice previously anesthetized with xylazine and ketamine (Bioflex AS-631, Cooner Wire, Chatsworth, CA). Electrodes were exteriorized at the back of the neck and protected by a plastic tube attached to the skin. Electrodes were connected to a Bio Amplifier, which was connected to an electromyogram acquisition system (ADInstruments, Colorado Springs, CO). A 10.5-mm-diameter balloon catheter was gently inserted into the colon at 5 mm proximal to the rectum (Fogarty arterial embolectomy catheter, 4F, Vygon). Ten-second distensions were performed at pressures of 15, 30, 45, and 60 mm Hg acquired by inflating the balloon in a stepwise fashion with water (20, 40, 60 and 80 μL respectively) with 5-min rest intervals.51 (link) Electromyographic activity of the abdominal muscles was recorded and visceromotor responses were calculated using Chart 5 software (ADInstruments).
7
Cystometric Evaluation of Bladder Function
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Under isoflurane anesthesia, a polyethylene catheter (PE-50, Clay-Adams, Parsippany, NJ, USA) was inserted into the bladder through the bladder dome, and the catheter was secured with a purse suture. Rats were then placed in restraining cages (W 80 mm × L 300 mm × H 150 mm, Yamanaka Chemical Ind., Ltd., Japan). After the recovery from anesthesia, animals were placed in the cages for at least 2 hours for acclimation, and bladder activity was recorded in the awake condition through the bladder catheter connected via a three-way stopcock to a pressure transducer and a pump to infuse saline solution at a rate of 0.04 ml/min. After rhythmic bladder contractions became stable for at least 60 min, the following cystometric parameters were measured; basal pressure (BP), micturition threshold (MT), intercontraction intervals (ICI), peak pressure (PP), voided volume (VV), residual volume (RV), voiding efficiency (VE), and bladder compliance. Chart 5 software (AD Instruments, Milford, MA, USA) was used for data analysis.
8
Fetal Hemodynamic Monitoring During Hypoxia-Ischemia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fetal mean arterial pressure (MAP) and heart rate (HR) were recorded throughout the experiment using pressure transducers with amplification, digitized (Power Lab, AD Instruments, Castle Hill, NSW, Australia) and recorded to a computer using Chart 5 software (AD Instruments). The MAP and HR were recorded in all fetuses for at least 24 h prior to the HI insult.
9
Visceromotor Response to Colorectal Distension
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Abdominal pain was assessed by measuring the visceromotor responses (VMRs) of mice to colorectal distension (CRD) following a previously described protocol17 ,35 . Briefly, electrodes were implanted in the abdominal external oblique muscles and exteriorized onto the back of the necks of mice at least 15 days prior to VMR measurement. Conscious mice were habituated to a plexiglass cylinder for 30 min per day for 3 consecutive days before VMR measurement to accustom the animals to partial constraint. For recording, the electrodes were connected to an electromyogram (EMG) acquisition system (AD Instruments). The colon was distended by inflating a balloon catheter inserted intra-anally such that it ended 1.5 cm proximal to the anus. The mice were subjected to four 10-second distensions (15, 40, and 65 mmHg) at 3-minute intervals. EMG activity was amplified and digitized using a transducer connected to a P511 AC amplifier (Grass Instruments) and a Powerlab device with Chart 5 software (AD Instruments). EMG activity was rectified, and responses were recorded as the increase in the area under curve (AUC) of the EMG amplitude during CRD versus the baseline period.
10
Vascular Reactivity Profiling of TEVs
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!