The N2ase enzyme was prepared from Av and reacted with CO using the same protocol from our previous studies.23 (link), 44 The infrared spectra were collected with a Bruker Vertex 70v FT-IR under cryogenic temperatures. Photolysis was induced by a broadband Sutter Instruments xenon-arc lamp. Samples were held in custom built cells with Teflon spacers to give a pathlength of 70 microns.
Vertex 70v ft ir
Manufactured by Bruker
Sourced in United States
The Vertex 70v FT-IR is a Fourier Transform Infrared Spectrometer designed for high-performance spectroscopic analysis. It features a vacuum-sealed optical bench to minimize atmospheric interference and enable precise measurements across a wide range of infrared wavelengths.
Automatically generated - may contain errors
8 protocols using vertex 70v ft ir
1
Cryogenic IR Analysis of N2ase-CO Interaction
2
Infrared Spectroscopy of Biocomposite Materials
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Infrared spectra of the
biocomposite materials were acquired by using an ATR accessory (MIRacle
ATR, PIKE Technologies) with a diamond crystal coupled to an FTIR
spectrometer (Vertex 70v FTIR, Bruker). All spectra were recorded
between 4000 and 600 cm–1, with a resolution of
4 cm–1, accumulating 128 scans.
biocomposite materials were acquired by using an ATR accessory (MIRacle
ATR, PIKE Technologies) with a diamond crystal coupled to an FTIR
spectrometer (Vertex 70v FTIR, Bruker). All spectra were recorded
between 4000 and 600 cm–1, with a resolution of
4 cm–1, accumulating 128 scans.
3
Probing Silk Fibroin Micro-Gel Nanostructure
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The nanostructure of SF µgels was examined with a cold field emission Scanning Electron Microscope (SEM Jeol JSM-7500FA, Tokyo, Japan). Briefly, SF µgels were dehydrated in graded ethanol series (50, 70, 90, 95, and 100%), then transferred into a critical point dryer (CPD, K850WM, Quorum Technologies, Lewes, UK) and rinsed 5 times with CO2 under supercritical conditions. Samples were mounted onto conductive carbon tape, gold-sputtered, and examined using an accelerating voltage of 10 kV. Fiber and pore dimensions were quantified on SEM images using ImageJ (https://imagej.nih.gov/ij/ ).
The chemical structure of SF gels was assessed by Fourier Transformed Infrared Spectroscopy (FTIR) performed on a Vertex 70 v FTIR (Bruker, MA, USA) equipped with a MIRacleATR accessory (PIKE Technologies, WI, USA). Spectra were measured in the range from 400 cm−1 to 4000 cm−1 with a resolution of 4 cm−1 and a 64 scan-acquisition. Quantification of SF secondary structure (β-sheets content) was performed by the self-deconvolution of the Amide I region according to the literature [35 (link), 38 (link)].
The chemical structure of SF gels was assessed by Fourier Transformed Infrared Spectroscopy (FTIR) performed on a Vertex 70 v FTIR (Bruker, MA, USA) equipped with a MIRacleATR accessory (PIKE Technologies, WI, USA). Spectra were measured in the range from 400 cm−1 to 4000 cm−1 with a resolution of 4 cm−1 and a 64 scan-acquisition. Quantification of SF secondary structure (β-sheets content) was performed by the self-deconvolution of the Amide I region according to the literature [35 (link), 38 (link)].
4
Characterization of Nanoparticle Morphology
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The morphology and particle size of hBN, B4C, hBN-ALA, and C4B-ALA were investigated using transmission electron microscopy (EOL JEM-ARM200CFEG UHR-TEM, Tokyo, Japan). Fourier transform infrared spectroscopy (Bruker VERTEX 70v. FTIR) was used to validate the interaction of hBN and C4B nanoparticles with ALA. Finally, all the samples were characterized by UV-vis spectroscopy (Epoch™, Biotek, Winooski, VT, USA).
5
Morphology and Properties of Au-Coated Foams
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Morphology
of foams coated with a layer of 10 nm Au was studied using a scanning
electron microscope (SEM) (JEOL JSM-6490LA) at an acceleration voltage
of 10 kV. The pore size distribution was characterized using Pascal
140 Evo and Pascal 240 Evo mercury intrusion porosimeters (Thermo
Fisher Scientific). The chemical composition and interactions of components
in the foams were studied using a single-reflection attenuated total
reflection (ATR) accessory (MIRacle ATR, PIKE Technologies) coupled
to a Fourier transform infrared (FTIR) spectrometer (Vertex 70v FT-IR,
Bruker). All spectra shown were averaged from 128 repetitive scans
recorded in the range from 3800 to 600 cm–1 with
a resolution of 4 cm–1. Thermal images were recorded
using an infrared camera (IR camera) FLIR A655sc, and subsequently
analyzed using ResearchIR software. The optical absorption spectra
(200–1800 nm) were obtained using a Cary JEOL UV–vis–NIR
spectrophotometer. The thermal decomposition profiles and the first
derivative curves were obtained through thermogravimetric analyses
using a Q500 analyzer with a heating rate of 10 °C min–1, from 30 to 800 °C in a N2 atmosphere. The water
contact angle was measured using a DataPhysics OCAH 200 contact angle
goniometer. Thermal conductivity measurements were conducted using
a TCi thermal conductivity analyzer.
of foams coated with a layer of 10 nm Au was studied using a scanning
electron microscope (SEM) (JEOL JSM-6490LA) at an acceleration voltage
of 10 kV. The pore size distribution was characterized using Pascal
140 Evo and Pascal 240 Evo mercury intrusion porosimeters (Thermo
Fisher Scientific). The chemical composition and interactions of components
in the foams were studied using a single-reflection attenuated total
reflection (ATR) accessory (MIRacle ATR, PIKE Technologies) coupled
to a Fourier transform infrared (FTIR) spectrometer (Vertex 70v FT-IR,
Bruker). All spectra shown were averaged from 128 repetitive scans
recorded in the range from 3800 to 600 cm–1 with
a resolution of 4 cm–1. Thermal images were recorded
using an infrared camera (IR camera) FLIR A655sc, and subsequently
analyzed using ResearchIR software. The optical absorption spectra
(200–1800 nm) were obtained using a Cary JEOL UV–vis–NIR
spectrophotometer. The thermal decomposition profiles and the first
derivative curves were obtained through thermogravimetric analyses
using a Q500 analyzer with a heating rate of 10 °C min–1, from 30 to 800 °C in a N2 atmosphere. The water
contact angle was measured using a DataPhysics OCAH 200 contact angle
goniometer. Thermal conductivity measurements were conducted using
a TCi thermal conductivity analyzer.
6
In situ FTIR Characterization of Catalysts
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In situ FTIR spectra of all samples were measured using an FTIR spectrometer (VERTEX 70v FTIR; Bruker, Billerica, MA, USA) [45 (link)] under operating conditions and accumulated 16 scans with a resolution of 4 cm−1 in the range of 4000–400 cm−1. The gas mixture of NH3 (500 ppm), NO (500 ppm), and O2 (5 vol.%) with N2 was used for in situ FTIR, and the flow rate was 0.3 L/min.
7
ATR-FTIR Spectroscopic Characterization
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Infrared spectra were recorded
with an ATR accessory (MIRacle ATR, PIKE Technologies) with a diamond
crystal coupled to a Fourier transform infrared (FTIR) spectrometer
(Vertex 70v FTIR, Bruker). All spectra were recorded from 4000 to
600 cm–1 with a resolution of 4 cm–1, accumulating 128 scans.
with an ATR accessory (MIRacle ATR, PIKE Technologies) with a diamond
crystal coupled to a Fourier transform infrared (FTIR) spectrometer
(Vertex 70v FTIR, Bruker). All spectra were recorded from 4000 to
600 cm–1 with a resolution of 4 cm–1, accumulating 128 scans.
8
Attenuated Total Reflection FTIR Spectroscopy
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Infrared spectra of samples were obtained with an attenuated total reflection (ATR) accessory (MIRacle ATR, PIKE Technologies) coupled to a Fourier transform infrared spectrophotometer FTIR spectrometer (Vertex 70v FT-IR, Bruker). All spectra were recorded in the range from 3800 to 600 cm−1, with 4 cm−1 resolution, accumulating 64 scans. The sample was gently placed on a spot of ATR accessory and slowly pressed, with the part grown in contact with the substrate (named “bottom”) on the ATR crystal. To ensure the reproducibility of the obtained spectra, three samples of each type are measured45 (link). Spectra analysis was performed with Origin pro 2016 software.
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