Dulbecco s modified eaglemedium dmem high glucose

In this experimental study, Dulbecco’s Modified Eagle
Medium (DMEM) high glucose and RPMI-1640 were
purchased from Biowest (Austria). Fetal bovine serum
(FBS) and penicillin- streptomycin were bought from Gibco
(Germany). Pure (98%) arbutin powder, 2, 4, 6-tripyridyls-
triazine (TPTZ), and 3- [4, 5-dimethylthiazol-2-yl]-2,
5-diphenyltetrazolium (MTT) were purchased from
Sigma-Aldrich (Germany). Annexin V-FITC apoptosis
detection kit was purchased from eBioscience (San
Diego, CA, USA). Tert-butyl hydroperoxide (t-BHP) was
obtained from MERK (Germany) and cDNA synthesis
Kit and YTA qPCR probe MasterMix 2x, were purchased
from Yekta Tajhiz (Iran).

Human fibrosarcoma (HT1080), cervical cancer (Hela), colorectal cancer (Caco-2), and human lung cancer cell lines (A549) were purchased from American Type Culture Collection (ECACC, Salisbury, UK). Cervical cancer cells (Hela) and fibrosarcoma cells (HT-1080) were maintained in a RPMI medium supplemented with 10% foetal bovine serum (FBS) and were expanded at 37 °C and 5% CO₂. Caco-2 and A549 cells were grown in Dulbecco’s modified eagle medium (DMEM)-high glucose (biowest, Riverside, MO, USA) enriched with (10% v/v) (foetal bovine serum) FBS (biowest, Riverside, MO, USA) and antibiotics (100 U/mL penicillin, 100 μg mL streptomycin) (Lonza, Basel, Switzerland). Cells were maintained at 37 °C in an incubator with 5% CO₂ atmosphere. Part of the experiments were carried out in CERRMA (Center of Excellence for Research in Regenerative Medicine and its Applications), Faculty of Medicine, Alexandria University.