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Q column

Manufactured by Shimadzu
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The Q column is a specialized chromatography column designed for the separation and purification of various chemical compounds. It functions as a stationary phase in liquid chromatography systems, providing efficient separation of target analytes from complex mixtures. The core function of the Q column is to facilitate the separation and isolation of desired compounds based on their interactions with the column's stationary phase material.

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Lab products found in correlation

Gc 2014 gas chromatograph, by Shimadzu (2 mentions) E2695, by Waters Corporation (1 mentions) Dr2800, by HACH (1 mentions) Fiveeasy ph meter, by Mettler Toledo (1 mentions) E2698 hplc system, by Waters Corporation (1 mentions) Rid 10a, by Shimadzu (1 mentions) Lc 20a, by Shimadzu (1 mentions)

4 protocols using q column

1

Analytical Methods for Wastewater Characterization

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The contents of TS, VS, C and N were analyzed using previously described methods26,27 (link) and pH was determined using a FiveEasy™ pH meter (METTLER TOLEDO, Switzerland). The soluble chemical oxygen demand (sCOD) concentration was measured using a spectrophotometer (Hach DR-2800, USA) and a Hach test kit. The sCOD was measured in the supernatant after filtration (0.45 μm filters). HPLC (Waters e2695) incorporating a refractive index detector was used to measure VFA contents. The column used in this analysis was Aminex HPX-87H (300 mm × 7.8 mm) with a mobile phase of 0.05 M H2SO4 and a flow rate of 0.5 ml min−1 at 50 °C.
A GC-2014 gas chromatograph (Shimadzu, Japan) equipped with a thermal conductivity detector and a Porapak Q column was used to determine the gas composition of (CH4, CO2 and N2). The carrier gas was Ar, and a flow rate of 20 ml min−1 was maintained for the analysis. The injection port, column and detector temperatures were 30 °C, 70 °C and 120 °C, respectively.
Xing T., Yu S., Zhen F., Kong X, & Sun Y. (2020). Anaerobic fermentation of hybrid Pennisetum mixed with fruit and vegetable wastes to produce volatile fatty acids. RSC Advances, 10(55), 33261-33267.
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2

Analytical Methods for Biogas Components

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pH was
determined using a FE28-Standard meter (Mettler-Toledo,
Zurich, Switzerland) with a glass electrode calibrated in buffers
at pH 7.0 and 4.0. VFAs were quantified using an e2698HPLC system
(Waters, Milford, CT) equipped with a column (Bio-Rad, Hercules, CA)
at 50 °C with 0.5 mM H2SO4 as the mobile
phase at a flow rate of 0.5 mL min–1. Methane (CH4) and carbon dioxide (CO2) contents were analyzed
using a GC-2014 gas chromatograph (Shimadzu, Kyoto, Japan) equipped
with a thermal conductivity detector (120 °C) and Porapak Q column
(70 °C); argon was used as the carrier gas (20 mL min–1).
Wang C., Li Y, & Sun Y. (2020). Acclimation of Acid-Tolerant Methanogenic Culture for Bioaugmentation: Strategy Comparison and Microbiome Succession. ACS Omega, 5(11), 6062-6068.
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3

Quantifying Metabolite Concentrations via HPLC and GC

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To determine the concentration of metabolites, 2 mL of culture supernatants that had been centrifuged at 10,000 rpm for 10 min was filtered through a 0.2-mm syringe filter before being pipetted into the chromatographic sample tube. The high-performance liquid chromatography (HPLC) (LC-20A, Shimadzu, Japan) was used to determine the concentrations of formate, lactate, acetate, pyruvate, acetoin, and succinate using a Shimadzu PREP-ODS(H) column and a Shimadzu RID-10A refractive index detector with 0.2% aqueous phosphoric acid at a flow rate of 0.8 mL/min. The GC system (GC-2010, Shimadzu, Japan) was used to determine the concentrations of alcohols using a Parapak Q column with He at a flow rate of 1 mL/min. The concentrations of analytes were calculated by contrasting the peak sizes to a standard curve constructed for each compound.
Lu P., Bai R., Gao T., Chen J., Jiang K., Zhu Y., Lu Y., Zhang S., Xu F, & Zhao H. (2024). Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production. Applied Microbiology and Biotechnology, 108(1), 146.
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4

Measuring N2O Partial Pressure in Lakes

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The partial pressure of N2O (pN2O) was measured from February to October 2012 during the nitrification project, and for the complete time span of the lake sentinel project using the same technique. pN2O measurements were obtained using headspace equilibration [35 (link)] where a 1.12 L glass bottle was filled using the overflow teachnique with lake water from both depths, hermetically sealed and 0.12 L of water was removed from the sealed bottle and replaced with ambient air. The bottle was mixed vigorously for 2 min to achieve headspace equilibration with water. Nine millilitres of air were then sampled in triplicate using an airtight syringe and transferred into 9 mL pre-evacuated glass vials capped with an airtight butyl seal. Ambient air samples were also collected. Samples were analyzed using a Shimadzu GC-2040 gas chromatograph, with a Poropaq Q column to separate gases. N2O concentrations were determined using an ECD detector. Concentration in the water (Cwater) and the expected saturation concentration in water at air equilibrium (Ceq) were corrected for before and after equilibrium sample temperature and ambient atmospheric pressure. N2O deviation from saturation (Δ N2O) was calculated as Cwater−Ceq. During the winter, we assumed no exchange with the atmosphere and used the Ceq of the first winter sample for the Δ N2O calculations.
Massé S., Botrel M., Walsh D.A, & Maranger R. (2019). Annual nitrification dynamics in a seasonally ice-covered lake. PLoS ONE, 14(3), e0213748.
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