Minicollect k3 edta tubes

Blood from mice was obtained by heart puncture and collected in EDTA tubes (Mini‐Collect K3EDTA tubes, Greiner AG). WBC count and hematocrit were measured using an animal blood counter (sciI Vet abc, Germany). For flow cytometry, erythrocytes were lysed using Gay's solution (10 mM KHCO₃ and 75 mM NH₄Cl, pH 7.4). Flow cytometry was used to determine the percentage of human CD45⁺ cells in the blood, lymph nodes, liver, and kidney (CD45 Monoclonal Antibody (HI30), APC, #17‐0459‐42, Thermo Fisher Scientific). Single‐cell suspensions were prepared by mincing organs through a 70 μm cell strainer (BD Biosciences, USA). All analyses were performed on a BD FACS Canto II™ instrument and calculated with FACSDiva software (v8.0.1, BD Biosciences).

Blood samples were collected in MiniCollect® K3EDTA tubes (Greiner Bio-One, Austria) for hematological analysis, which included the determination of hemoglobin concentration (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cells (WBC), and red blood cells (RBC; SYSMEX XT-4000i™ automated hematology analyzer, Sysmex America, Inc.). The plasma levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea and glucose were determined using COBAS® 6000 automated analyzer (Roche Diagnostics, England). Additionally, the glutathione peroxidase (GPX) activity was estimated using Ransel Glutathione Peroxidase kit (Randox Laboratories, United Kingdom), according to the manufacturer's instructions.