Lamin b antibody

M‐CSF and RANKL were purchased from Peprotech. Alpha‐MEM was purchased from Gibco. FBS was purchased from Access Biologicals. TRAP staining solution kit was purchased from Sigma‐Aldrich. Cell Counting Kit‐8 (CCK‐8) solution was purchased from Dojindo. LPA and S1P receptor antagonists (W146, JTE013, VPC23019, VPC24191 and VPC32183) were purchased from Cayman. PTX was purchased from Calbiochem. All antibodies for phospho‐MAPKs were purchased from Cell Signaling Technology. NFATc1 antibody and total MAPKs antibodies were purchased from Santa Cruz Biotechnology. Lamin B antibody was purchased from Abcam.

AF was isolated from a fungal strain of Ascochyta viciae and was kindly provided by the co-authors Dr Young-Chae Chang, Dae-gu, South Korea and Dr Junji Magae, Tokyo, Japan. The phytopathogenic fungus Ascochyta viciae LIBERT was isolated from the agricultural field and deposited at the Mage Bioinstitute, Tokyo, Japan. AF was isolated as a hypolipidemic substance from the fermented broth of the strain [21 (link)]. The basic structure of AF is a prenyl-phenolic compound. LPS (Escherichia coli 0111:B4), Hoechst staining solution, Griess reagent, antibodies against β-actin, iNOS, COX-2, p-ERK, p38, p-p38, AP-1, NF-κB, and p65, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, USA). JNK and p-JNK antibodies were obtained from Cell Signaling Technology, Inc. (Beverly, MA, USA). ERK antibody was purchased from Upstate (Atlanta, GA, USA). Lamin B antibody was purchased from ABcam (Cambridge, UK). The EMSA kit was obtained from Promega, Inc. (Madison, WI, USA).