Sodium alginate (Na-Alg, viscosity of 1% solution at 20 °C of 300–400 mPa s), barium chloride (BaCl2) dihydrate, and calcium chloride (CaCl2) dihydrate were obtained from Wako Pure Chemical (Osaka, Japan). RGD peptide-coupled alginate (NOVATACH LVM GRGDSP peptide-coupled alginate; RGD-Alg) was obtained from FMC BioPolymer (Sandvika, Norway). Fetal bovine serum (FBS), 1 M HEPES buffer, LIVE/DEAD Viability/Cytotoxicity kit, green fluorescent microbeads (Φ of 1.0 μm and 3.1 μm), Prolong Gold antifade reagent with DAPI, and Alexa Fluor 488 goat anti-rabbit IgG were obtained from Thermo Fisher Scientific (MA, USA). Polydimethylsiloxane (PDMS) prepolymer (Silpot 184) was obtained from Dow Corning Toray (Tokyo, Japan). (Heptadecafluoro-1,1,2,2-tetrahydrodecyl) triethoxysilane (fluorosilane) was obtained from Gelest (PA, USA). Poly-l -lysine (PLL; average Mv of 150 000–300 000), Dulbecco's modified Eagle's medium (DMEM), penicillin–streptomycin solution, trypsin/EDTA solution, PKH26 red fluorescent cell linker kit, PKH67 green fluorescent cell linker kit, and alginate lyase (from Flavobacterium multivorum) were obtained from Sigma Aldrich (MO, USA). Rabbit polyclonal anti-elastin antibody was obtained from Abcam (Cambridge, UK). All other chemicals were of analytical grade.
Calcium chloride cacl2 dihydrate
Manufactured by Fujifilm
Sourced in Japan
Calcium chloride (CaCl2) dihydrate is a chemical compound that is commonly used in laboratory equipment. It is a crystalline solid that is hygroscopic in nature, meaning it readily absorbs moisture from the air. The main function of calcium chloride dihydrate is to act as a desiccant, which is a substance that removes or absorbs water from the surrounding environment.
Automatically generated - may contain errors
Lab products found in correlation
Sodium alginate na alg, by Fujifilm (2 mentions)
Alginate lyase from flavobacterium multivorum, by Merck Group (2 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Green fluorescent microbeads, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin solution, by Merck Group (2 mentions)
Rabbit polyclonal anti elastin antibody, by Abcam (1 mentions)
Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions)
Hepes buffer 1 m, by Thermo Fisher Scientific (1 mentions)
Pkh26 red fluorescent cell linker kit, by Merck Group (1 mentions)
Pkh67 green fluorescent cell linker kit, by Merck Group (1 mentions)
Poly l lysine (pll), by Merck Group (1 mentions)
Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (1 mentions)
Trypsin edta solution, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Rockland Immunochemicals (1 mentions)
Gelatin, by Fujifilm (1 mentions)
Glutaraldehyde solution, by Fujifilm (1 mentions)
Phosphate buffered saline (pbs), by Takara Bio (1 mentions)
Hemoglobin from bovine blood, by Merck Group (1 mentions)
2 protocols using calcium chloride cacl2 dihydrate
1
Regenerative Engineered Tissue Constructs
2
Alginate-Gelatin Hydrogel Fabrication
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Sodium alginate (Na-alg; viscosity of 300–400
mPa s at 1% solution at 20 °C), gelatin (from bovine bone; pH
of a 50 g L–1 solution at 35 °C of 5.5–6.0,
catalog number of 076-02765), calcium chloride (CaCl2)
dihydrate, 25% glutaraldehyde solution, and trisodium citrate dihydrate
were obtained from Fujifilm Wako Pure Chemical (Osaka, Japan). Dextran
(from Leuconostoc spp., Mr of 450,000–650,000), Dulbecco’s modified
Eagle’s medium (DMEM), penicillin–streptomycin solution,
trypsin/ethylenediaminetetraacetic acid (EDTA) solution, alginate
lyase (from Flavobacterium multivorum),
and hemoglobin (from bovine blood) were obtained from Sigma-Aldrich
(MO, USA). Polydimethylsiloxane (PDMS) prepolymer (Silpot 184) was
obtained from Dow Corning Toray (Tokyo, Japan). Bovine serum albumin
(BSA) was obtained from Rockland Immunochemicals (PA, USA). Phosphate-buffered
saline was obtained from Takara Bio (Shiga, Japan). Fetal bovine serum
(FBS), green fluorescent microbeads (diameter of 0.5 μm), and
alamarBlue cell viability reagent were obtained from Thermo Fisher
Scientific (MA, USA). These materials were used as received. All other
chemicals were of analytical grade.
mPa s at 1% solution at 20 °C), gelatin (from bovine bone; pH
of a 50 g L–1 solution at 35 °C of 5.5–6.0,
catalog number of 076-02765), calcium chloride (CaCl2)
dihydrate, 25% glutaraldehyde solution, and trisodium citrate dihydrate
were obtained from Fujifilm Wako Pure Chemical (Osaka, Japan). Dextran
(from Leuconostoc spp., Mr of 450,000–650,000), Dulbecco’s modified
Eagle’s medium (DMEM), penicillin–streptomycin solution,
trypsin/ethylenediaminetetraacetic acid (EDTA) solution, alginate
lyase (from Flavobacterium multivorum),
and hemoglobin (from bovine blood) were obtained from Sigma-Aldrich
(MO, USA). Polydimethylsiloxane (PDMS) prepolymer (Silpot 184) was
obtained from Dow Corning Toray (Tokyo, Japan). Bovine serum albumin
(BSA) was obtained from Rockland Immunochemicals (PA, USA). Phosphate-buffered
saline was obtained from Takara Bio (Shiga, Japan). Fetal bovine serum
(FBS), green fluorescent microbeads (diameter of 0.5 μm), and
alamarBlue cell viability reagent were obtained from Thermo Fisher
Scientific (MA, USA). These materials were used as received. All other
chemicals were of analytical grade.
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