The largest database of trusted experimental protocols

Dodecyltrimethylammonium bromide dtab

Manufactured by Merck Group
Sourced in United States

Dodecyltrimethylammonium bromide (DTAB) is a cationic surfactant used in various laboratory applications. It is a white crystalline powder that is soluble in water and other polar solvents. DTAB is commonly used as a detergent, emulsifier, and wetting agent in scientific research and industrial processes.

Automatically generated - may contain errors

5 protocols using dodecyltrimethylammonium bromide dtab

1

Synthesis of Laponite-Based Formulations

Check if the same lab product or an alternative is used in the 5 most similar protocols
Laponite® (Na+[(Si8Mg5.5Li0.3)O20(OH)4]−0.7) was obtained from Southern Clay Products (Gonzales, TX). Pluronic F68 and dodecyltrimethylammonium bromide (DTAB) were purchased from Sigma, and sodium dodecyl sulfate was purchased from Fisher Chemical. Mineral oil was purchased from Sigma-Aldrich. All chemicals were used as received.
+ Open protocol
+ Expand
2

Cobalt(II) Determination Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals used are of at least analytical reagent grade. Cobalt chloride hexahydrate (CoCl2·6H2O), metal ions studied for interferences, pyrocatechol violet and nitric acid were obtained from Carlo-Erba, France. 1-Undecanol, 1-dodecanol, dodecyltrimethyl ammonium bromide (DTAB), trimethyltetradecyl ammonium bromide (TTAB), and cethyltrimethyl ammonium bromide (CTAB) were purchased from Sigma-Aldrich (USA). 1-Octanol was obtained from Panreac (Spain). Methanol (HPLC grade) and acetonitrile (HPLC grade) were obtained from Merck (Germany). Acetone was purchased from Q-rac (Malaysia). Sodium sulphate anhydrous was purchased from Fluka (Japan). Sodium chloride, sodium hydrogen phosphate and sodium dihydrogen phosphate were purchased from Ajax Finechem (Australia). Aluminium chloride was obtained from Ajax Finechem Pty Ltd (New Zealand). All solutions were prepared using deionized water with the resistivity of 18.2 MΩ cm from RiOs™ Type I Simplicity 185 (Millipore Waters, USA). Stock solution of standard Co(ii) 100 mg L−1 (or 1.695 mmol L−1) was prepared and used throughout.
+ Open protocol
+ Expand
3

Graphite-based Nanocomposite Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Graphite (>98% purity) was purchased from Loba Chemie, India. Sulfuric acid (98%), hydrochloric acid (35%), sodium nitrate (98% purity), potassium permanganate (99% purity) and hydrogen peroxide (30% w/v) were purchased from Rankem Laboratories, India. Solutions for small-angle neutron scattering (SANS) measurements were prepared in heavy water (99.9% purity) procured from Tokyo Chemical Industry, India. P103 (EO17-PO60-EO17) was received as a gift sample from BASF Corp., USA. Dodecyltrimethyl ammonium bromide (DTAB, >99% purity) was purchased from Sigma-Aldrich, USA. All other reagents were of analytical grade and used as received.
+ Open protocol
+ Expand
4

Quantifying Cellular NAD+ and NADH Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
To evaluate qualitative values of NAD+, we used NADH, NAD/NADH-Glo Assay (Promega), according to the manufacturer’s recommendations. Briefly, 1×105 cells were lysed with 400 μl of PBS and then 400 μl of 0.2N NaOH containing 1% Dodecyltrimethylammonium bromide (DTAB) (Sigma-Aldrich) was added. To measure NAD+, 100 μl of 0.4N HCl were added to the lysed cell samples (200 μl) and then heated at 60C for 15 min. After 10 min incubation at room temperature, 0.5M Trizma base buffer (100 μl, Sigma-Aldrich) was added. To measure NADH, lysed cell samples (200 μl) were heated at 60C for 15 min, and then incubated at room temperature for 10 min, followed by adding equal volume of HCl/Trizma solution. Finally, samples were seeded into 96 well plates and incubated with NAD/NADH Glo detection reagent for 60 min. Data were compared to DMSO treated cells and expressed as % control.
+ Open protocol
+ Expand
5

Synthesis of Emulsion Polymers using Cationic Surfactants

Check if the same lab product or an alternative is used in the 5 most similar protocols
The monomers used were styrene (St) and methyl methacrylate (MMA) (Sigma-Aldrich 99% purity for each monomer, Saint Louis, MO, USA). St and MMA were passed through a column (Sigma-Aldrich, Saint Louis, MO, USA) to remove the inhibitors. V-50 (2,2’-azobis(2-methylpropionamidine) dihydrochloride) from Sigma-Aldrich (97% purity, Saint Louis, MO, USA) was used as initiator. The surfactants to stabilize the EPs were C1EG (>95% purity) from IoLiTec (Heilbronn, Baden-Wurtemberg, Germany) and dodecyltrimethylammonium bromide (DTAB, ≥98% purity) from Sigma-Aldrich (Saint Louis, MO, USA). Both surfactants were used without further purification. Nitrogen gas (N2, 99.99% purity, Infra, Saltillo, Mexico) was used to deplete oxygen from the reaction systems and to maintain an inert atmosphere in the reactor. In all reactions, purified and deionized water was used (Milli-Q filtration system, Molsheim, Bas-Rhin, Grand Est, France). The inhibitor to stop the EP reactions in the withdrawn aliquots was hydroquinone (Aldrich, 98% purity; Saint Louis, MO, USA), 1% w/w aqueous solution. Figure 1 shows the molecular structure of the C1EG, DTAB, and [C12mim]Cl cationic surfactants.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!