Anti atg5

Manufactured by Boster Bio

Anti-Atg5 is a primary antibody that specifically recognizes the autophagy-related protein 5 (Atg5). Atg5 is a key component of the autophagy machinery and plays a crucial role in the initiation and progression of the autophagy process. The Anti-Atg5 antibody can be used to detect and quantify Atg5 expression in various cell and tissue samples, allowing researchers to study the regulation and function of autophagy in different biological systems.

Automatically generated - may contain errors

Lab products found in correlation

Anti β actin, by Boster Bio (1 mentions) Anti gapdh, by Boster Bio (1 mentions) Anti lc3, by Boster Bio (1 mentions) Anti p62, by Boster Bio (1 mentions) Anti mtor, by Cell Signaling Technology (1 mentions) Rabbit anti ampk, by Cell Signaling Technology (1 mentions) Rabbit anti bax, by Cell Signaling Technology (1 mentions) Cisplatin, by Merck Group (1 mentions) Caspase 9, by Cell Signaling Technology (1 mentions) Hoechst 33258 staining kit, by Beyotime (1 mentions) Anti p62, by Merck Group (1 mentions) Anti gsk3β, by Cell Signaling Technology (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti suv39h1, by Proteintech (1 mentions) Anti igf 1r, by Merck Group (1 mentions) Anti lc3b, by Proteintech (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti akt c67e7, by Cell Signaling Technology (1 mentions) Anti calnexin, by Santa Cruz Biotechnology (1 mentions) Anti glut3, by Proteintech (1 mentions)

2 protocols using anti atg5

Ginseng Compound G‐Rb3 Protects Against Cisplatin‐Induced Toxicity

Check if the same lab product or an alternative is used in the 5 most similar protocols

G‐Rb3 (purity ≥ 98.5%, HPLC method) was isolated and purified from the leaves of Panax quinquefolium (American ginseng). Cisplatin was purchased from Sigma Chemicals with purity more than 99%. Compound C, rapamycin (Ram) and acetylcysteine (NAC) also were purchased from MedChemExpress Biotech and stored at −80°C in darkness. The commercial assay kits for determining reduced glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), blood urea nitrogen (BUN) and creatinine (CRE) were bought from Nanjing Jiancheng Biological Research Institute. Haematoxylin and eosin (H&E) dying kit and Hoechst 33258 staining kit were obtained from Beyotime Co, Ltd. The immunohistochemically assay kits together with SABC‐DyLight488 immunofluorescence staining kits were obtained from BOSTER Biological Technology Co, Ltd. The primary rabbit monoclonal antibodies including anti‐LC3, anti‐BNIP3, anti‐β‐actin, anti‐GAPDH, anti‐Atg3, anti‐Atg5, anti‐Atg7 and anti‐p62 were all provided by BOSTER Biological Technology Co, Ltd. The rabbit anti‐AMPK, rabbit anti‐mTOR, rabbit anti‐Bax, Bcl‐2, Bad, caspase 3 and caspase 9 were acquired from Cell Signaling Technology. TUNEL commercial kit was purchased from Roche Applied Science. All other reagents and chemicals, unless indicated, were obtained from Beijing Chemical Factory.

Xing J., Hou J., Ma Z., Wang Z., Ren S., Wang Y., Liu W., Chen C, & Li W. (2019). Ginsenoside Rb3 provides protective effects against cisplatin‐induced nephrotoxicity via regulation of AMPK‐/mTOR‐mediated autophagy and inhibition of apoptosis in vitro and in vivo. Cell Proliferation, 52(4), e12627.

+ Open protocol

+ Expand

Western Blotting for Autophagy and Metabolism Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

For western blot analysis, cell were lysed in RIPA buffer (50mM Tris-HCl [pH 7.4], 1% NP40, 0.25% sodium deoxycholate, 150 mM NaCl, 1 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, 1 μg/mL aprotinin, and 1 μg/mL leupeptin) for 10 minutes at 4°C. Lysates were centrifuged at 14,000 × g for 10 minutes at 4°C, and proteins were separated by gel electrophoresis. The antibodies used in this study were anti-LC3B (Proteintech Group, Rosemont, IL), anti-ATG5 (Boster, Pleasanton, CA), anti-p62 (Sigma), anti-β-actin (Santa Cruz Biotechnology, Santa Cruz, CA), anti-GLUT1 (Proteintech Group), anti-GLUT3 (Proteintech Group), anti-insulin receptor β (Proteintech Group, Rosemont, IL), anti-IGF-1R (Millipore, Temecula, CA), anti-GAPDH (Santa Cruz Biotechnology), anti-calnexin (Santa Cruz), anti-SUV39H1 (Proteintech Group), anti-GSK-3β-phospho-serine 9 (Cell Signaling) and anti-GSK-3β (Cell Signaling), anti-AKT (C67E7, Cell Signaling), and anti-AKT phospho-serine 473 (Cell Signaling). Western blots were performed at least in triplicate.

Craven R.J., Frazier H, & Thibault O. (2021). Dependence of glucose transport in neuronal model systems on autophagy and GAPDH (glyceraldehyde-3 phosphate dehydrogenase) activity. Brain research, 1776, 147747.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!