Anti myc

Antibodies against phosphorylated and total IRF3, IKKε, TBK1, NF-κB p65, ERK, JNK, and p38 were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-TRAF3, anti-β-actin, and HRP-conjugated secondary antibodies were from Santa Cruz. Anti-Myc, anti-Flag, anti-HA, and anti-GFP were from Origene. Anti-PINK1, anti-Parkin, and anti-YAP1 were from Abcam Biotechnology (Cambridge, UK). Anti-K48-ubiquitin and anti-K63-ubiquitin were from Millipore (Kenilworth, USA).

Primary antibodies used were: anti-zDHHC15 (Abcam, Cambridge, MA, #ab121203, 1:250, western blot), anti-zDHHC15 (Thermo Scientific, Waltham, MA, #PA39327, immunofluorescence, 1:100), anti-Flag (Sigma Aldrich, St Louis, MO, #F3165, 1:1000), anti-Myc (Origene, Rockville, MD, #TA150121, 1:1000), anti-β-actin (Novus Biologicals, Centennial, CO, #NB600-503, 1:1000), anti-PSD-95 (Abcam, Cambridge, MA, #ab2723, 1:500), anti-gephyrin (Synaptic Systems, Göttingen, Germany #147011, 1:500), anti-GAD65 (Synaptic Systems, Göttingen, Germany #198104, 1:500), anti-GFAP (Synaptic Systems, Göttingen, Germany #173004, 1:400), anti-MAP2 (Millipore, Burlington, MA, MB3418, 1:2000), anti-giantin (Synaptic Systems, Göttingen, Germany, #263004, 1:500), anti-GM130 (BD Biosciences, Franklin Lakes, NJ, #610822, 1:200). Secondary antibodies from Life Technologies (Carlsbad, CA,) used: (1:500), anti-mouse AF568 IgG2a (#A21134), anti-mouse AF647 IgG1 (#21240), anti-rabbit AF488 (#A11008), anti-rabbit AF568 (#A11011), anti-guinea pig AF633 (#A21105). HRP conjugated secondary antibodies were obtained from Bio-Rad (Hercules, CA): goat anti-mouse #170-6516 and goat anti-rabbit (#170-6515, 1:300). DAPI was used for nuclear staining (Life Technologies, Carlsbad, CA, #D1306, 1:1000).