Fluorescence images were acquired with a Leica-Microsystems DM2500 microscope. Optical microscope images were obtained with a Zeiss Axioplan 2 microscope, equipped with a MediaCybernetics Evolution VF digital camera. For images of the shape change this microscope was equipped with a Linkam LTS420 thermal stage under nitrogen atmosphere, using heating rates ranging from 3 to 10 °C min−1. The movie of the “kicking” glass bead was taken with a Leica WILD M10 microscope, equipped with an Evolution MP5.0 Leica DMC2900 digital camera with frame rate of 30 fps and a Linkam LTS420 thermal stage under nitrogen atmosphere.
Axioplan 2 microscope
Manufactured by Media Cybernetics
Sourced in United States
The Axioplan 2 microscope is a high-performance optical microscope designed for advanced imaging applications. It features a sturdy, ergonomic design and a range of sophisticated optical components to provide clear, high-resolution images. The core function of the Axioplan 2 is to magnify and visualize small-scale specimens for detailed observation and analysis.
Automatically generated - may contain errors
2 protocols using axioplan 2 microscope
1
Microscopic Imaging of Material Transformations
2
Quantitative Analysis of Neuronal Activation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sections were viewed using a Zeiss Axioplan 2 microscope equipped for fluorescence imaging, and Image Pro-Plus (v5.1, Media Cybernetics, Rockville, MD, USA) was used for image capture and analysis. Fos, pERK and pp38 immunohistochemistry was examined in seven sections at 120-μm intervals from 1800 μm caudal to 1080 μm caudal to obex. All slides were blinded prior to experimental analysis, and all quantification was undertaken by the same investigator and repeated on 10% of samples on a different day. These repeat measurements were within a 5% margin of the first count.
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