Anti tissue factor antibody

Heparin was purchased from Sigma (St. Louis, MO, USA) and TNF-α was purchased from Abnova (Taipei, Taiwan). The molecular weight of Heparin used as positive control in our experiments is 3500. Anti-tissue factor (TF) antibody, rivaroxaban, and argatroban were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Thromboxane A₂ (TXA₂) analog and U46619 were purchased from Calbiochem-Novabiochem Corp. (San Diego, CA, USA). Prothrombin, thrombin, Factor VIIa, X, and Xa were purchased from Haematologic Technologies (Essex Junction, VT, USA) and APTT-XL and thromboplastin-D were purchased from Fisher Diagnostics (Middletown, VA, USA). S-2222 and S-2238 were purchased as substrate of FXa and thrombin from ChromogenixAB (Mölndal, Sweden). Aggregometer (Chronlog, Havertown, PA, USA), thrombotimer (Behnk Elektronik, Norderstedt, Germany), microplate reader (Tecan Austria GmbH, Grödig, Austria), and spectrophotometer (TECAN, Männedorf, Switzerland) were used.

Freeze-dried Oxya chinensis sinuosa (5 kg) were obtained from the National Academy of Agricultural Science, RDA, Korea, and identified by one of the authors (M-A. Kim). A voucher specimen (CNU-INS 201603) was deposited at the Pharmacognosy Laboratory of the College of Pharmacy, Chungnam National University, Daejeon, Korea. TNF-α was purchased from Abnova (Taipei, Taiwan). The anti-tissue factor (TF) antibody was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The thromboxane A2 (TXA2) analog, U46619, was purchased from Calbiochem-Novabiochem Corp. (San Diego, CA, USA). Factors VIIa, X, and Xa, and thrombin were obtained from Haematologic Technologies (Essex Junction, VT, USA). Recombinant tissue factor pathway inhibitor (TFPI) was obtained from Amercian Diagnostica Inc (Stamford, CT, USA). The aPTT assay reagent and PT reagents were purchased from Fisher Diagnostics (Middletown, VA, USA); the chromogenic substrate (S-2222) was purchased from Chromogenix AB (Mölndal, Sweden). Collagen and tirofiban were purchased from Sigma (St. Louis, MO, USA). All other reagents were of the highest commercially available grade.