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Tk luc

Manufactured by Promega
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The TK-luc is a luciferase-based reporter gene that can be used to monitor transcriptional activity in living cells. It contains the firefly luciferase gene under the control of the herpes simplex virus type 1 thymidine kinase (HSV-1 TK) promoter.

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Lab products found in correlation

Nfat luc, by Promega (2 mentions) Creb1 luc, by Promega (2 mentions) Gata4 luc, by Promega (2 mentions) Mef2 luc, by Addgene (2 mentions) Veritas 96 well microplate luminometer, by Promega (2 mentions) Xfect reagent, by Takara Bio (2 mentions) Dual luciferase assay kit, by Promega (2 mentions) Passive lysis buffer (plb), by Promega (2 mentions)

Close spelling variants of this product

PRL-TK Renilla-Luc plasmid REN-Luc/pRL-TK PRL-TK renilla luciferase (pRL-TK Luc), PRL-TK-luc PRL-TK-Luc Renilla luciferase

2 protocols using tk luc

1

Transcription Factor Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNCMs were transfected with NFAT-luc, CREB1-luc, GATA4-luc, TK-luc (Promega) or MEF2-luc (Addgene), and using X-fect reagent (Clontech) according to manufacturer’s protocol. After 24 hours transfection, cells were stimulated with 25 μM of PE for 6 hours, lysates harvested using passive lysis buffer (Promega) and luciferase activity determined using the Dual Luciferase assay kit (Promega) and Veritas 96 well microplate luminometer (Turner Biosystems) following manufacturer’s protocol.
Lee D.I., Zhu G., Sasaki T., Cho G.S., Hamdani N., Holewinski R., Jo S.H., Danner T., Zhang M., Rainer P.P., Bedja D., Kirk J.A., Ranek M.J., Dostmann W.R., Kwon C., Margulies K.B., Van Eyk J.E., Paulus W.J., Takimoto E, & Kass D.A. (2015). Phosphodiesterase 9A Controls Nitric-oxide Independent cGMP and Hypertrophic Heart Disease. Nature, 519(7544), 472-476.
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2

Transcription Factor Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNCMs were transfected with NFAT-luc, CREB1-luc, GATA4-luc, TK-luc (Promega) or MEF2-luc (Addgene), and using X-fect reagent (Clontech) according to manufacturer’s protocol. After 24 hours transfection, cells were stimulated with 25 μM of PE for 6 hours, lysates harvested using passive lysis buffer (Promega) and luciferase activity determined using the Dual Luciferase assay kit (Promega) and Veritas 96 well microplate luminometer (Turner Biosystems) following manufacturer’s protocol.
Lee D.I., Zhu G., Sasaki T., Cho G.S., Hamdani N., Holewinski R., Jo S.H., Danner T., Zhang M., Rainer P.P., Bedja D., Kirk J.A., Ranek M.J., Dostmann W.R., Kwon C., Margulies K.B., Van Eyk J.E., Paulus W.J., Takimoto E, & Kass D.A. (2015). Phosphodiesterase 9A Controls Nitric-oxide Independent cGMP and Hypertrophic Heart Disease. Nature, 519(7544), 472-476.
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