Gfap chicken polyclonal antibody

The following dilutions were used: NG2-rabbit polyclonal antibody (EMD Millipore) at 1:1000 (Western), 1:200 (immunofluorescence/immunohistochemistry) and 1:100 (flow cytometry); PDGFRα at 1:500, PDGFRβ at 1:50 (Cell Signaling Technology); Ki67 (Abcam) at 1:200; GFAP-chicken polyclonal antibody (Abcam) at 1:1000 (immunofluorescence) and 1:100 (flow cytometry), Olig2-mouse monoclonal (EMD Millipore) at 1:200 (immunofluorescence) and 1:100 (flow cytometry). HRP secondary antibodies (Kirkergaard and Perry laboratories) at 1:5000 dilution for western blotting and 1:200 dilution for immunohistochemistry, all Alexa labeled antibodies (Life Technologies) at 1:500 dilution. For flow cytometry, 1 μg of Alexa 488 or Alexa 647 labeled antibodies and 0.25 μg of PE cy 5.5 in 100 μl of sample was used.

SFN was purchased from Aladdin (China). The CCK-8 assay kit was purchased from Dojindo (Japan). FITC Annexin V Apoptosis Detection Kit I was purchased from BD Biosciences (USA). DNase I was purchased from Sigma (USA). The TUNEL apoptosis detection kit and DAPI were purchased from Beyotime (China). DMEM/F12 medium, fetal bovine serum (FBS), 25% (w/v) trypsin-EDTA, Phosphate Buffered Saline (PBS), goat serum, Alexa fluor 594 labeled goat anti-chicken secondary antibodies and Alexa fluor 488 labeled goat anti-rabbit secondary antibodies, RIPA lysis buffer, protein phosphatase inhibitors, BCA protein assay kit, and the ECL reagent kit were purchased from Thermo Scientific (USA). Caspase-3 and cleaved Caspase-3 rabbit polyclonal antibodies, SB203580, p38 MAPK, and phospho-p38 MAPK rabbit mAb were purchased from Cell Signaling Technology (USA). GFAP chicken polyclonal antibody, GFAP rabbit polyclonal antibody, and AQP4 rabbit polyclonal antibody were purchased from Abcam (USA). Beta tubulin rabbit polyclonal antibody and horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies were purchased from Proteintech (USA).
SFN was dissolved in dimethyl sulfoxide (DMSO) to a storage concentration of 100 mM. SB203580 was dissolved in dimethyl sulfoxide (DMSO) to a storage concentration of 10 mM. 0.01%(v/v) DMSO was used as vehicle.