Culture reagents were from Gibco (Grand Island, NY, USA). ADSCs were cultured in a conventional medium that consisted of Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with 100-U/mL penicillin G sodium, 100-mg/mL streptomycin sulfate, and 10% vol/vol FBS. Unless otherwise stated, all the other reagents were from Sigma (St. Louis, MO, USA). VPA was from Suju (Guangzhou, China). RG108, Reprogramming Cocktail Set I and purmorphamine were from Biovision (San Francisco, USA). Cell Counting Kit-8 (CCK-8) was from Dojindo (Kyushu, Japan). Cell Cycle and Apoptosis Analysis Kit and Annexin V-FITC/PI apoptosis detection kit were from KeyGEN (Nanjing, China). Monoclonal anti-vimentin (NeoMarkers) was from Lab Vision Corp (Fremont, MO, USA). Goat anti-CD34 polyclonal antibody, goat anti-mouse IgG and goat anti-rabbit IgG were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). EZgeneTM Tissue RNA Miniprep Kit was from Biomiga (San Diego, CA, USA). ReverTra Ace qPCR RT Kit, Blend Taq and Blend Taq-Plus were from Toyobo (Osaka, Japan). Primers were synthetized by BGI (Shenzhen, China).
Culture reagents
Manufactured by Thermo Fisher Scientific
Sourced in United States
Culture reagents are a type of laboratory equipment used to provide the necessary nutrients and growth factors for the cultivation and maintenance of cell cultures. These reagents support the survival, proliferation, and differentiation of cells in a controlled and optimal environment.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Revertra ace qpcr rt kit, by Toyobo (1 mentions)
Goat anti rabbit igg, by Santa Cruz Biotechnology (1 mentions)
Goat anti mouse igg, by Santa Cruz Biotechnology (1 mentions)
Blend taq, by Toyobo (1 mentions)
Ezgene tissue rna miniprep kit, by Biomiga (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Keygen Biotech (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Blend taq plus, by Toyobo (1 mentions)
Goat anti cd34 polyclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Cell cycle and apoptosis analysis kit, by Keygen Biotech (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
β actin antibody, by Novus Biologicals (1 mentions)
Horseradish peroxidase conjugated second antibody, by Cell Signaling Technology (1 mentions)
Phospho irf7, by Cell Signaling Technology (1 mentions)
Antibodies against phospho stat1, by Cell Signaling Technology (1 mentions)
Lyovec, by InvivoGen (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Poly dg dc, by InvivoGen (1 mentions)
12 protocols using culture reagents
1
Adipose-Derived Stem Cell Culture
2
ELISA Assay for TNF-α and IL-6 Quantification
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The enzyme-linked immunosorbent assays (ELISA) kit for TNF-α and IL-6 were from eBioscience (San Diego, CA, USA). DMEM, FBS, and all culture reagents were purchased from Gibco BRL (Life technologies, USA). Antibodies against p38, phosphorylated p38, JNK, phosphorylated JNK, ERK, phosphorylated ERK, α-tubulin, iNOS, phosphorylated NFκB p65 were purchased from Santa Cruz (Biotechnology, Inc., USA). β-actin antibody was from Novus Biologicals (Littleton, CO, USA). Antibodies against phosphorylated IκBα, phosphorylated STAT1 were purchased from Cell Signaling Technologies (Boston, MA, USA). Lipopolysaccharide (LPS, from E. coli, 0127: B8), gelatin, dexamethasone (Dex) and other chemicals were purchased from Sigma Chemicals, Co. (St Louis, Mo, USA).
3
Innate Immune Signaling Pathway Components
4
MTT Assay for PC12 Cell Viability
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Pheochromocytoma PC12 cells, derived from rat adrenal medulla, were obtained from American Type Culture Collection (Manassas, VA) and cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 6% fetal bovine serum and horse serum, 100 units/mL penicillin and 100 μg/mL streptomycin in a humidified CO2 (7.5%) incubator at 37°C. Fresh medium was applied every other day. Culture reagents were from Invitrogen (Carlsbad, CA). Cell viability was assessed by MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] assay. Cells were plated in 96-well plate for 24 hours and treated with drugs for 48 hours before adding MTT. Then the cells were incubated with MTT for another 3 hours at 37°C. After that, absorbance of 570 nm was measured in a microplate reader (Thermo Fisher Scientific, Waltham, MA) [10 (link)].
5
Mouse iPSCs Culture Protocol
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Mouse iPSCs were grown on mitomycin C-treated MEF feeders in standard ESC medium containing 10% KSR. All culture reagents were obtained from Invitrogen unless otherwise stated.
6
Isolation of Porcine Nerve-Derived Cells
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The PNs used for cell isolation were obtained from common iliac nerve segments harvested after brain death from human organ donors. This study was reviewed and approved by the Institutional Review Board of Inje University Busan Paik Hospital. Porcine skin-derived collagen (Matrixen®-PSC) and collagenase type I were obtained from SK Bioland (Chunan, Korea) and Worthington Biochemical (Lakewood, NJ, USA), respectively. Growth factors, including recombinant epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), β-nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and transforming growth factor-β1 (TGF-β1), were purchased from Peprotech (Seoul, Korea). Culture media, animal sera, and culture reagents were obtained from Invitrogen (Seoul, Korea), unless otherwise specified.
7
Colon Cancer Cell Line Cultivation
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Reagents and cell culture. 5-FU was purchased from Sigma-aldrich (St. Louis, MO, USa). Mouse antibodies against FOXK1 (G-4), cyclin D1 (A-12), CDK4 (H-22), CDK6 (C-21), caspase 3 p11 (C-6), caspase 8 p18 (E-8), caspase 9 p35 (A-9), PARP-1 (H-300), GAPDH (G-9), horseradish peroxidase-conjugated anti-goat IgG, and anti-mouse IgG were purchased from Santa cruz Biotechnology (Santa cruz, ca, USa). culture reagents were purchased from Invitrogen (carlsbad, ca, USa). The colon cancer cell lines SW480 and SW1116 were obtained from the American Type Culture Collection (ATCC; Rockville, Md, USa) and were cultured as previously described (25) . The cells were maintained at 37˚c with 5% cO 2 in a humidified incubator and were subcultured using 0.25% trypsin every 2-3 days, before confluence was reached.
8
Calbindin Overexpression in MN9D Cells
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The establishment and characterization of MN9D cells that stably overexpress calbindin-D28K (MN9D/Calbindin) or vector control (MN9D/Neo) were described elsewhere [11 (link)]. Both MN9D parental cells and MN9D/Neo cells had no detectible levels of endogenous calbindin, as determined by immunoblot analysis [11 (link)]. Cells were plated at a density of 2×104 cells/well in 48-well Corning® Costar® plates (coated with 25 µg/ml poly-D-lysine. Cultures were maintained in complete culture medium (CCM), consisting of Dulbecco's Modified Eagle's Medium supplemented with 10% (v/v) heat-inactivated fetal bovine serum and 250 µg G418/ml, for 3 d at 37℃ in an incubator with an atmosphere of 10% CO2. Culture medium was subsequently changed to serum-free N2 medium containing experimental reagents and further incubated for various time periods. Culture reagents were from Life Technologies (Grand Island, NY). Staurosporine was obtained from Sigma-Aldrich (St. Louis, MO, USA) and BAPTA from Calbiochem (La Jolla, CA, USA).
9
Establishment and Cultivation of ESCC Cell Lines
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In-house developed ESCC cell lines SLMT-1, HKESC-1, and HKESC-2 were used as before [18 (link),19 (link)]. ESCC cell lines KYSE-150, KYSE-180, KYSE-270, KYSE-410, and KYSE-450 purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany) were cultured accordingly. Cultured cells were maintained in a humidified atmosphere at 37°C with 5% CO2. Culture reagents were obtained from Life Technologies (Waltham, MA).
10
Culturing Mouse Macrophage RAW264.7 Cells
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The mouse macrophage RAW264.7 cell line was purchased from the American Type Culture Collection (Rockville, MD, USA). Cells were cultured in high-glucose Dulbecco's modified Eagle's medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) that contained 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) with penicillin (100 U/ml) and streptomycin sulfate (100 µg/ml) at 37°C in an incubator with 5% CO2. Culture reagents were purchased from Gibco (Thermo Fisher Scientific, Inc.).
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