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Immunoprecipitation buffer

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Immunoprecipitation buffer is a solution used in the process of immunoprecipitation, which is a technique used to isolate and purify specific proteins or protein complexes from a mixture. The buffer helps maintain the structural integrity and functionality of the target proteins during the immunoprecipitation process.

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Lab products found in correlation

Dynabeads protein a, by Thermo Fisher Scientific (1 mentions) Anti m6a antibody, by Synaptic Systems (1 mentions)

Spelling variants of this product

Radio-immunoprecipitation assay (RIPA) lysis buffer (18 citations) RIPA (Radio-Immunoprecipitation Assay) buffer (9 citations) RNA immunoprecipitation lysis buffer (9 citations) RIP immunoprecipitation buffer (4 citations) RNA immunoprecipitation (RIP) lysis buffer (2 citations) Radio‐immunoprecipitation assay cell lysis buffer (2 citations)

2 protocols using immunoprecipitation buffer

1

Immunoprecipitation and Protein Analysis

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SGC7901/OXA-E2F1, MGC803/OXA-E2F1, SGC7901/OXA-GFP and MGC803/OXA-GFP cells were harvested from 1640 culture medium and then supplemented with an immunoprecipitation buffer (Sigma-Aldrich, USA). The supernatant of the culture medium was collected and immunoprecipitated with an anti-c-MYC or anti-GFP antibody and the supernatant was separated by SDS-PAGE. Protein expression was analyzed by immunoblotting.
Yan L.H., Chen Z.N., L.i.-.L.i., Chen J., Wei W.E., Mo X.W., Qin Y.Z., Lin Y, & Chen J.S. (2016). miR-135a promotes gastric cancer progression and resistance to oxaliplatin. Oncotarget, 7(43), 70699-70714.
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2

m6A RNA Immunoprecipitation and Quantification

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A portion (10%) of RNA was obtained for use as an input sample. RNA was immunoprecipitated with anti-m6A antibody (Synaptic Systems) in immunoprecipitation buffer (Sigma, USA) at 4°C for 2 h and then incubated with Dynabeads protein A (Invitrogen, USA) at 4°C for 2 h. The bound RNA was eluted twice by competition with m6A 5′-monophosphate sodium salt at 4°C for 1 h. Following ethanol precipitation, the input RNA and immunoprecipitated m6A RNAs were determined by quantitative real-time PCR analysis as before.
Cheng C., Wu Y., Xiao T., Xue J., Sun J., Xia H., Ma H., Lu L., Li J., Shi A., Bian T, & Liu Q. (2020). METTL3-mediated m6A modification of ZBTB4 mRNA is involved in the smoking-induced EMT in cancer of the lung. Molecular Therapy. Nucleic Acids, 23, 487-500.
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