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Mouse t cell kit

Manufactured by Lonza
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The Mouse T cell kit is a laboratory product designed for the isolation and purification of T cells from mouse samples. It provides the necessary components and protocols to effectively separate and collect T cells from various mouse tissue sources. The kit enables researchers to obtain purified mouse T cell populations for downstream applications and analyses.

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Lab products found in correlation

Nucleofector 2b, by Lonza (2 mentions) Pcmv6 mchat mchat orf in a pcmv6 kan neo plasmid, by OriGene (2 mentions)

Spelling variants of this product

Nucleofector Kits for Mouse T Cells (3 citations)

2 protocols using mouse t cell kit

1

Monoclonal Jurkat Cell Line Generation

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Jurkat cells (originally obtained from ATCC) were a generous gift from Dr. Charles Chu, The Feinstein Institute for Medical Research, Manhasset, NY, U.S.A. pCMV6-mChAT (mChAT ORF in a pCMV6-kan/neo plasmid (ORIGene, Rockville, MD (MC220061)) was nucleofected into Jurkat cells with a mouse T cell kit (Lonza, Allendale, NJ VPA-1006) and a Nucleofector 2b (Lonza). Transformed cells were selected over 2 weeks with G418 (400 mg/mL) in RPMI containing 10% FBS. Isolated cells were individually selected by pipet and serial dilution for monoclonal populations, then grown for an additional month to ensure stable chromosomal integration. Monoclonal lines were analyzed for ChAT expression by Western blotting. Jurkat cell lines tested negative for Mycoplasma sp., EBV, HAdV, Hantaan, HCMV, Hepatitis A, Hepatitis B, Hepatitis C, HHV 6, HHV 8, HIV1, HIV2, HSV 1, HSV 2, HTLV 1, HTLV 2, LCMV, Seoul, Sin Nombre, VZV.
Olofsson P.S., Steinberg B.E., Sobbi R., Cox M.A., Ahmed M.N., Oswald M., Szekeres F., Hanes W.M., Introini A., Liu S.F., Holodick N.E., Rothstein T.L., Lövdahl C., Chavan S.S., Yang H., Pavlov V.A., Broliden K., Andersson U., Diamond B., Miller E.J., Arner A., Gregersen P.K., Backx P.H., Mak T.W, & Tracey K.J. (2016). Blood pressure regulation by CD4+ lymphocytes expressing choline acetyltransferase. Nature biotechnology, 34(10), 1066-1071.
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2

Monoclonal Jurkat Cell Line Generation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Jurkat cells (originally obtained from ATCC) were a generous gift from Dr. Charles Chu, The Feinstein Institute for Medical Research, Manhasset, NY, U.S.A. pCMV6-mChAT (mChAT ORF in a pCMV6-kan/neo plasmid (ORIGene, Rockville, MD (MC220061)) was nucleofected into Jurkat cells with a mouse T cell kit (Lonza, Allendale, NJ VPA-1006) and a Nucleofector 2b (Lonza). Transformed cells were selected over 2 weeks with G418 (400 mg/mL) in RPMI containing 10% FBS. Isolated cells were individually selected by pipet and serial dilution for monoclonal populations, then grown for an additional month to ensure stable chromosomal integration. Monoclonal lines were analyzed for ChAT expression by Western blotting. Jurkat cell lines tested negative for Mycoplasma sp., EBV, HAdV, Hantaan, HCMV, Hepatitis A, Hepatitis B, Hepatitis C, HHV 6, HHV 8, HIV1, HIV2, HSV 1, HSV 2, HTLV 1, HTLV 2, LCMV, Seoul, Sin Nombre, VZV.
Olofsson P.S., Steinberg B.E., Sobbi R., Cox M.A., Ahmed M.N., Oswald M., Szekeres F., Hanes W.M., Introini A., Liu S.F., Holodick N.E., Rothstein T.L., Lövdahl C., Chavan S.S., Yang H., Pavlov V.A., Broliden K., Andersson U., Diamond B., Miller E.J., Arner A., Gregersen P.K., Backx P.H., Mak T.W, & Tracey K.J. (2016). Blood pressure regulation by CD4+ lymphocytes expressing choline acetyltransferase. Nature biotechnology, 34(10), 1066-1071.
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