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K2edta microtainers

Manufactured by BD
Sourced in United States, Italy

K2EDTA microtainers are laboratory collection devices used for obtaining small volume blood samples. They contain the anticoagulant K2EDTA, which prevents the blood from clotting during collection and storage.

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2 protocols using k2edta microtainers

1

Comprehensive Hematological and Biochemical Profiling

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All blood samples were collected in animals under i.p. anesthesia (20 μl/g.b.w. of 1.2% Avertin-2,2,2-tribromoethanol, 2.4% 2-methyl-2-butanol; Sigma-Aldrich, Italy). For determination of hematological parameters, 20 μl of whole blood was collected in K2EDTA microtainers (Becton, Dickinson and Company, USA) and the samples were analyzed using the commercially available automated cell counter “Simply cell” (BPC BioSed s.r.l., Italy). For cytomorphological examination, each sample from peripheral blood smears was prepared using the differential staining Diff-Quick (Dade SpA, Italy) and analyzed under optical microscopy. For determination of clinical chemistry parameters, blood samples were collected in SST microtainers (Serum Separator Tube; Becton, Dickinson and Company) and centrifuged in a microcentrifuge (5415R model; Eppendorf s.r.l., Italy) at 13,000 rpm for 7 min to separate the serum. Cholesterol (CHOL), triglycerides (TRI), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), blood urea nitrogen (BUN) and lactate dehydrogenase (LDH) were measured using the automatic analyzer Keylab (BPC BioSed s.r.l., Rome, Italy).
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2

Haematological and Clinical Chemistry Analysis

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For determination of haematological parameters, 20 μL of whole blood were collected in K2 EDTA microtainers (Becton Dickinson, Milan, Italy) and the samples were analysed using the commercially available automated cell counter “Drew3” (BPC BioSed, Rome, Italy). Haematological parameters [red blood cell (RBC) counts, haemoglobin (HGB), haematocrit (HCT), white blood cell (WBC) total and relative differential counts as lymphocytes (LYMF), mid-range cells (MID), granulocytes (GRAN), mean corpuscular haemoglobin (MCH), mean corpuscular volume (MCV), mean corpuscular haemoglobin concentration (MCHC) and platelet counts (PLT), red cell distribution width (RDW), mean platelet volume (MPV)] were analysed.
For determination of clinical chemistry parameters, blood samples were collected in SST microtainers (Serum Separator Tube; Becton Dickinson, Milan, Italy) and centrifuged in a microcentrifuge (5415R model; Eppendorf, Italy) at 13,000 rpm for 7 min to separate the serum. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN) and creatinine (CRE) were measured using the automatic analyser Keylab (BPC BioSed. Rome, Italy).
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