Mouse anti bovine type 2 collagen igg antibody assay kit

Manufactured by Chondrex

Sourced in United States

The Mouse anti‐bovine type II collagen IgG antibody assay kit is a laboratory product that detects and measures the levels of mouse anti-bovine type II collagen IgG antibodies in samples. It serves as a tool for researchers to quantify the presence of these specific antibodies.

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Lab products found in correlation

Mouse anti mouse type 2 collagen igg antibody assay kit, by Chondrex (2 mentions) Imark microplate reader, by Bio-Rad (1 mentions)

Spelling variants of this product

Bovine type II collagen (124 citations) Type II collagen (20 citations) Bovine collagen II (5 citations) Mouse anti-mouse type II collagen IgG antibody assay kit (3 citations) Collagen II (3 citations) Mouse anti-mouse type II collagen IgG antibody assay (2 citations)

5 protocols using mouse anti bovine type 2 collagen igg antibody assay kit

Measuring Inflammatory Cytokines and Autoantibodies

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Concentrations of IL‐1β, IL‐6, TNF‐α and IL‐17A in sera or culture supernatants were measured using an ELISA kit from R&D Systems. Serum concentration of anti‐type II collagen IgG in mice was assessed using mouse anti‐bovine type II collagen IgG antibody assay kit (Chondrex). The absorbance of each well was measured with an iMark microplate reader (Bio‐Rad Laboratories, Hercules, CA, USA).

Tenshin H., Teramachi J., Ashtar M., Hiasa M., Inoue Y., Oda A., Tanimoto K., Shimizu S., Higa Y., Harada T., Oura M., Sogabe K., Hara T., Sumitani R., Maruhashi T., Sebe M., Tsutsumi R., Sakaue H., Endo I., Matsumoto T., Tanaka E, & Abe M. (2022). TGF‐β‐activated kinase‐1 inhibitor LL‐Z1640‐2 reduces joint inflammation and bone destruction in mouse models of rheumatoid arthritis by inhibiting NLRP3 inflammasome, TACE, TNF‐α and RANKL expression. Clinical & Translational Immunology, 11(1), e1371.

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Quantifying Collagen Autoantibodies in Mice

Cited 1 time

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Circulating levels of mouse anti-collagen antibodies (auto-antibodies) and bovine anti-collagen antibodies was assessed by ELISA in serum collected from mice in the CIA model. A mouse anti-mouse type II collagen IgG antibody assay kit and mouse anti-bovine type II collagen IgG antibody assay kit was used, according to the manufacture's protocol (Chondrex Inc. Redmond, WA, USA). The antibody concentrations in the samples were calculated by comparison with the optical density (OD) values of standard anti-CII antibody (units/ml) provided in each of the assay kits as previously described (24 (link), 26 (link)).

Hemshekhar M., Piyadasa H., Mostafa D., Chow L.N., Halayko A.J, & Mookherjee N. (2020). Cathelicidin and Calprotectin Are Disparately Altered in Murine Models of Inflammatory Arthritis and Airway Inflammation. Frontiers in Immunology, 11, 1932.

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Cytokine and Collagen-Specific Antibody Quantification

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The concentrations of cytokines (IL‐21, IL‐17A, IL‐6, IL‐10, TNF‐α, or IFN‐γ) were measured using ELISA kits (Multisciences), and the titer of collagen‐specific antibody was analyzed using a mouse anti‐bovine type II collagen IgG antibody assay kit (Chondrex). All measurements followed kit protocols as described in the manufacturer's instructions.

Li J., Jin J., Li S., Zhong Y., Jin Y., Zhang X., Xia B., Zhu Y., Guo R., Sun X., Guo J., Hu F., Xiao W., Huang F., Ye H., Li R., Zhou Y., Xiang X., Yao H., Yan Q., Su L., Wu L., Luo T., Liu Y., Guo X., Qin J., Qi H., He J., Wang J, & Li Z. (2022). Tonsillar Microbiome‐Derived Lantibiotics Induce Structural Changes of IL‐6 and IL‐21 Receptors and Modulate Host Immunity. Advanced Science, 9(30), 2202706.

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Cytokine and Antibody Quantification

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The concentrations of cytokines (IL-6 and IL-10) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Multisciences), and the titer of collagen-specific antibody was analyzed using a mouse anti-bovine type II collagen IgG antibody assay kit (Chondrex). All measurements followed kit protocols as described in the manufacturer’s instructions.

He J., Chu Y., Li J., Meng Q., Liu Y., Jin J., Wang Y., Wang J., Huang B., Shi L., Shi X., Tian J., Zhufeng Y., Feng R., Xiao W., Gan Y., Guo J., Shao C., Su Y., Hu F., Sun X., Yu J., Kang Y, & Li Z. (2022). Intestinal butyrate-metabolizing species contribute to autoantibody production and bone erosion in rheumatoid arthritis. Science Advances, 8(6), eabm1511.

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Measuring Autoimmune Collagen Antibodies

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Serum levels of mouse anti-collagen antibodies (autoantibodies) and bovine anti-collagen antibodies (antibodies to the immunizing antigen) were determined by ELISA using a Mouse Anti-mouse Type II Collagen IgG Antibody Assay Kit and Mouse Anti-Bovine Type II Collagen IgG Antibody Assay Kit, respectively, according to the manufacture’s protocol (Chondrex Inc. Redmond, WA, USA). The antibody concentrations in the test samples were calculated by comparison with the optical density (OD) values of standard anti-CII antibody (units/μl).

Hemshekhar M., Anaparti V., El-Gabalawy H, & Mookherjee N. (2021). A bioavailable form of curcumin, in combination with vitamin-D- and omega-3-enriched diet, modifies disease onset and outcomes in a murine model of collagen-induced arthritis. Arthritis Research & Therapy, 23, 39.

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