Western blot analysis was performed using standard procedures. The following primary antibodies were used in the experiments: anti-FOXM1 antibody (Santa Cruz), anti-pAKT antibody (Peprotech, USA), anti-AKT antibody (Cell Signaling Technology, Beverly, MA, USA), anti-pGSK3β antibody (Proteintech) at 1:1000, anti-Snai1 antibody (Cell Signaling Technology), anti-pIGF1R antibody (Abcam, Cambridge, UK), anti-IGF1R antibody (Abcam, Cambridge, UK), anti-E-cadherin antibody (BD Biosciences, USA), anti-Vimentin antibody (Cell Signaling Technology), anti-N-cadherin antibody (Cell Signaling Technology), anti-pERK1/2 antibody (Abcam), anti-ERK1/2 antibody (Abcam), anti-HIF1α antibody (Novus Biologicals, USA), anti-ETS1 antibody (Abcam) and anti-β-actin antibody (Sigma, St. Louis, MO,USA).
Anti igf1r antibody
Manufactured by Abcam
Sourced in United Kingdom
Anti-IGF1R antibody is a lab equipment product that detects the presence and measures the levels of insulin-like growth factor 1 receptor (IGF1R) protein in biological samples. It can be used for research purposes in various applications such as western blotting, immunohistochemistry, and flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Anti vimentin antibody, by Cell Signaling Technology (1 mentions)
Anti foxm1 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti e cadherin antibody, by BD (1 mentions)
Anti ets1 antibody, by Abcam (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Anti n cadherin antibody, by Cell Signaling Technology (1 mentions)
Anti erk1 2 antibody, by Abcam (1 mentions)
Anti hif 1α antibody, by Novus Biologicals (1 mentions)
Anti akt antibody, by Cell Signaling Technology (1 mentions)
Anti perk1 2 antibody, by Abcam (1 mentions)
Anti snai1 antibody, by Cell Signaling Technology (1 mentions)
Ecl immunoblotting kit, by Merck Group (1 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Anti gapdh antibody, by Abcam (1 mentions)
3 protocols using anti igf1r antibody
1
Comprehensive Western Blot Analysis
2
Protein Expression Analysis in HCC
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After transfection, total proteins were extracted from HCC tissues and cells using RIPA lysis buffer (Beyotime, Beijing, China). Protein concentrations were calculated by BCA Protein Assay Kit (Beyotime, Beijing, China). Then, equal amount of protein samples were separated by 10% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membrane (Invitrogen). Next, membrane was blocking with 5% non-fat milk for 1 hour at room temperature and incubated with primary antibodies (anti-IGF1R antibody, 1:1000, Abcam; anti-GAPDH antibody, 1:10 000 Abcam) overnight at 4°C. In addition, the membrane was incubated with corresponding secondary antibodies at room temperature for 1 hour. The immunoreactive signal was detected by an ECL immunoblotting kit (Millipore, USA).
3
Western Blot Analysis of IGF1R and Akt
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Cells were collected in lysis buffer, and protein was extracted and analyzed by western blotting as described previously [18 (link)]. The antibodies used include anti-IGF1R antibody (1 : 500; Abcam) and anti-phosphorylated Akt antibody (Ser473; 1 : 2000; Cell Signaling Technology).
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