1260 uplc system

The samples were analyzed by an Agilent 1260 UPLC system consisting of an on-line degasser, a quaternary pump, an auto-sampler, and a column temperature controller. The chromatographic column was Waters Cortecs® T3 column (2.1 mm × 100 mm, 2.7 µm). The mobile phase contained 0.1% formic acid in water (A) and acetonitrile (B) with a flow rate of 0.4 mL/min using a gradient program as follows: 0–2% B from 0 to 5 min, 2%–5% B from 5 to 10 min, 5%–10% B from 10 to 15 min, 10%–13% B from 15 to 20 min, 13%–18% B from 20 to 24 min, 18%–24% B from 24 to 32 min and 24%–90% B from 32 to 40 min. And the injection volume of the sample and mixed standard solution was 3 μL.
The Agilent 1260 UPLC system was connected to a Triple TOF™ 5600 (AB SCIEX, Foster City, CA) with an ESI interface. Mass range was set at m/z 50–2000. The optimum parameters of MS/MS detector were set as follows: ion spray voltage was 4500 V for positive ion mode and -4500 V for negative ion mode; ion source temperature was 550 °C; ion source gas 1 was 50 psi; ion source gas 2 was 50 psi; curtain gas was 30 psi; collision energy was 10 V in MS mode and 45 V in MS² mode; and declustering potential was all set at 80 V. Peak View® Software V. 2.2 was used for data collection and processing.

Cannabinoid assays were run on an Agilent Technologies 1260 UPLC system (Santa Clara, CA) equipped with a G4212A DAD, G1316C temperature-controlled column compartment, G4226A autosampler, and G4204A quaternary pump. Cannabinoids were separated on a Poroshell 120 EC-C18 column with a Poroshell 120 EC-C18 guard column. The UPLC runs OpenLab CDS ChemStation Rev C. 01.06 (61) software (Agilent Technologies). Terpenoid analyses were carried out on an Agilent 7890B GC/7697A Headspace/5977A mass spectrophotometer with a DB-624UI and Agilent 5181–8818 split/splitless liner. Injector port temperature was 250°C with a transfer line, valve oven, and needle temperature of 180°C. Carrier gas was helium at a flow of 33.0 cm/sec. The mass spectrometer detector was set to scan with a range from 50 to 300 m/z. The instrument was controlled by Agilent MassHunter Quantitative Analysis (Vers. B.08.00 Build 8.0.593.0). Certified reference standards were from Restek (Bellefonte, PA) with MassHunter library confirmation.