Genesys tm 10s uv vis spectrophotometer

TPC of the MD2-PPC extracts was performed according to a method by Hossain and Rahman [40 (link)]. First, 200 µL of each extract was transferred into a test tube. Then, 0.2 mL of Folin–Ciocalteu solution was added, and the tube was vortexed. After 4 min, 1 mL of 15% sodium carbonate (Na₂CO₃) solution was added to the mixture and it was left to stand in the dark for 2 hours at room temperature. The blank solution was prepared in the same manner, except without the addition of extracts. The absorbance of mixtures was read at 765 nm against the blank using a Thermo Scientific GENESYS^TM 10S UV–Vis Spectrophotometer (Waltham, MA, USA). The gallic acid stock solutions were used to generate the standard calibration curve of different gallic acid concentrations (0, 25, 50, 100, 150, and 250 µg/mL). The total phenolic content was measured as milligrams of gallic acid equivalents (GAE)/100 g of FW using Equation (6) below: $$\mathrm{TPC}\left(\mathrm{mg}\frac{\mathrm{GAE}}{100\mathrm{g}}\right)={\mathrm{C}}_1\times \mathrm{DF}\times \frac{v}{m}$$
where TPC is the total phenolic content in mg GAE/100 g, C₁ is the concentration of gallic acid obtained from the standard curve in mg/mL, DF is the dilution factor, v is the volume of extract in mL, and m is the weight of the plant extract in grams.

The glucose concentration was recorded with a GlucoMen^® AERO 2K personal glucose meter (A. Menarini Diagnostics, Barcelona, Spain) with a dynamic range of 20 to 600 mg/dL of glucose (1–33 mM). Glucose test strips incorporate glucose oxidase (GOD) as the enzyme employed to oxidize glucose and [Fe(CN)₆]³⁻ as the redox mediator.
Incubation steps were conducted under gentle agitation in a Dynabeads^TM MX 12-tube Mixing Wheel (1.5 mL tubes containing ≥0.5 mL of solution were used with this vertical rotator), and magnetic separation was performed with a magnet incorporating a sample rack with 16 positions for standard 1.5 mL microcentrifuge tubes (DynaMag^TM-2), both purchased from Thermo Fisher Scientific (Madrid, Spain). Temperature control during the enzymatic hydrolysis step was carried out in a ThermoMixer Comfort (Eppendorf, Madrid, Spain). Solutions were homogenized by shaking with an IKA^®VORTEX Genious 3 (Merck, Madrid, Spain).
The spectrophotometric measurements (Trinder method) were performed with a GENESYS^TM 10S UV-Vis spectrophotometer (Thermo Scientific, Madrid, Spain), using disposable plastic cuvettes with an optical path length of 1 cm.