Sulforaphane

Manufactured by LGC

Sourced in Macao, Canada

Sulforaphane is a naturally occurring compound found in certain cruciferous vegetables, such as broccoli and cabbage. It is a versatile laboratory reagent that can be used in various research applications.

Automatically generated - may contain errors

Lab products found in correlation

Dylight 488 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Sicam 1, by R&D Systems (1 mentions) Svcam 1, by R&D Systems (1 mentions) Se selectin, by R&D Systems (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Bradford reagent, by Merck Group (1 mentions) Protease inhibitor cocktail tablet, by Roche (1 mentions)

2 protocols using sulforaphane

Inflammatory Pathway Modulation Assay

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Calcein O, where O = -diacetate tetrakis (acetoxymethyl) ester (calcein AM)), RPMI-1640; and DMEM medium were purchased from Life Technologies (Grand Island, NY). Enzyme-linked immunosorbent assay (ELISA) kits for human and mouse soluble adhesion molecules ICAM-1 (sICAM-1), VCAM-1 (sVCAM-1) and E-selectin (sE-Selectin) and mouse chemokines MCP-1/JE and KC ELISA kits for the determination of human IL-8 and MCP-1 were from R&D Systems (Minneapolis, MN). Goat anti-rabbit IgG, DyLight^™ 488 conjugated secondary antibody and Goat anti-rabbit HRP-IgG secondary antibody were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). NF-κB p65, VCAM-1 and F4/80 primary antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA), Santa Cruz Biotechnology (Santa Cruz, CA, USA) and BMA Biomedicals (Augst, Switzerland), respectively. Sulforaphane was from Toronto Research Chemicals (Toronto, CA, ≥98%, HPLC) and other general chemicals were from Sigma-Aldrich (St. Louis, MO).

Nallasamy P., Si H., Babu P.V., Pan D., Fu Y., Brooke E.A., Shah H., Zhen W., Zhu H., Liu D., Li Y, & Jia Z. (2014). Sulforaphane reduces vascular inflammation in mice and prevents TNF-α-induced monocyte adhesion to primary endothelial cells through interfering with the NF-κB pathway. The Journal of nutritional biochemistry, 25(8), 824-833.

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Sulforaphane Modulates Nrf2 Signaling

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Sulforaphane was purchased from Toronto Research Chemicals (Toronto, Canada). Sodium/Mc selenite, DMSO, BSO, and Bradford reagent were all purchased from Sigma (Dorset, UK). Protease inhibitor cocktail tablets were obtained from Roche Applied Science (UK). RPMI-1640 medium was purchased from Invitrogen Corporation (UK). Antibodies against Nrf2 (Catalog No.13032), Sam68 (Catalog No.333), COX-2 (Catalog No.376861), UGT (Catalog No.271268), β-actin (Catalog No.7210) were all purchased from Santa Cruz Biotechnology (Santa Cruz, Heidelberg, Germany). The nuclear Extraction Kit was purchased from Active Motif^® International (UK). Electrophoresis and Western blotting supplies were obtained from Bio-Rad (Hertfordshire, UK). The HiPerfection Transfection Reagent Kit was purchased from QIAGEN^® (west Sussex, UK), Nrf-2 siRNA (ID 115764) was obtained from Applied Biosystems (Manchester, UK).

He C., Buongiorno L.P., Wang W., Tang J.C., Miceli N., Taviano M.F., Shan Y, & Bao Y. (2021). The Inhibitory Effect of Sulforaphane on Bladder Cancer Cell Depends on GSH Depletion-Induced by Nrf2 Translocation. Molecules, 26(16), 4919.

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