Siinfekl mhc 1

Manufactured by Thermo Fisher Scientific

SIINFEKL/MHC-I is a recombinant protein that contains the SIINFEKL peptide sequence derived from the ovalbumin protein. It is commonly used as a model antigen for studying CD8+ T cell responses in mice.

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MHC-I (10 citations) SIINFEKL (8 citations)

2 protocols using siinfekl mhc 1

Antigen Presentation in B16 Cells

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B16 or B16-OVA cells were co-cultured with MutuDC1s or MutuDC2s as triplicates in a U-bottom 96-well plate in a CO₂ incubator for 1, 3 and 6 hours. Some of the 3 hours cultures were supplemented with a standard dose of PolyG/EDTA. After incubation the cells were washed, stained with eBioscience’s Fixable Viability Dye eFluor780, I-A/I-E (M5/114.15.2) and SIINFEKL/MHC-I (eBio25-D1.16) and analyzed by flow cytometer.

Herbst C.H., Bouteau A., Menykő E.J., Qin Z., Su Q., Buelvas D.M., Gyenge E., Mabbott N.A, & Igyártó B.Z. (2023). Dendritic Cells Overcome Cre/Lox Induced Gene Deficiency by Siphoning Material From Neighboring Cells Using Intracellular Monitoring—a Novel Mechanism of Antigen Acquisition. bioRxiv.

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B16 and MutuDC Antigen Presentation

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B16 or B16-OVA cells were co-cultured with MutuDC1s or MutuDC2s as triplicates in a U-bottom 96-well plate in a CO₂ incubator for 1, 3 and 6 h. Some of the 3 h cultures were supplemented with a standard dose of PolyG/EDTA. After incubation the cells were washed, stained with eBioscience’s Fixable Viability Dye eFluor780, I-A/I-E (M5/114.15.2) and SIINFEKL/MHC-I (eBio25-D1.16) and analyzed by flow cytometer.

Herbst C.H., Bouteau A., Menykő E.J., Qin Z., Gyenge E., Su Q., Cooper V., Mabbott N.A, & Igyártó B.Z. (2024). Dendritic cells overcome Cre/Lox induced gene deficiency by siphoning cytosolic material from surrounding cells. iScience, 27(3), 109119.

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