Ganciclovir

Manufactured by MedChemExpress

Sourced in United States

Ganciclovir is a synthetic guanine derivative with antiviral properties. It is used as a laboratory reagent for research purposes.

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Lab products found in correlation

3 protocols using ganciclovir

Screening Membrane Transporter Inhibitors for HCMV

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MRC5 and NHDF (ATCC, Manassas, VA) were cultured in DMEM (Corning #10–013-CV) with 10% FBS, 1mM HEPES (Corning, #25–060-CI), 100U/mL penicillin and 100g/mL streptomycin (Corning, #30–002-CI). The ARPE-19 human retinal epithelial cells (ATCC #CRL-2302) were cultured in DMEM/F-12 medium (Gibco, # 11765–054) at 1:1 ratio with FBS, HEPES and Pen/Strep. The HCMV strain AD169^IE2-YFP and a repaired AD169 (denoted BADrUL131-C4) containing the UL131-UL128 open reading frame of the HCMV strain TR and expresses the reporter EGFP (AD169^BADrUL131) (Wang, D. and T. Shenk, 2005 (link)) were propagated as described (Gardner, T.J. et al., 2013 ). Infectious virus yield was assayed on MRC5 by median tissue culture infective dose (TCID50). The Membrane Transporter/Ion Channel Compound Library (#HY-L011, 123 compounds (10mM DMSO) was from MedChem Express (Manmouth Junction, NJ). P-glycoprotein inhibitors elacridar (HY-50879), tariquidar (HY-10550), valspodar (#HY-17384), and zosuquidar (HY-15255) and ganciclovir (HY-13637) from MedChem Express were resuspended in DMSO (10mM).

Parsons A.J., Cohen T., Schwarz T.M., Stein K.R., Ophir S.I., Casado J.P, & Tortorella D. (2021). Valspodar limits human cytomegalovirus infection and dissemination. Antiviral research, 193, 105124.

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Antiviral Drug Screening Protocol

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Acetaminophen (catalog number HY-66005), arbidol hydrochloride (HY-14904A), aspirin (HY-14654), atazanavir sulfate (HY-17367A), baloxavir marboxil (HY-109025), daclatasvir (HY-10466), darunavir (HY-17040), dolutegravir (HY-13238), efavirenz (HY-10572), elbasvir (HY-15789), etravirine (HY-90005), galidesivir (BCX4430, HY-18649A), ganciclovir (HY-13637), glecaprevir (HY-17634), hydroxychloroquine (HY-B1370), indomethacin (HY-14397), ledipasvir (HY-15602), lopinavir (HY-14588), losartan (HY-17512), nafamostat mesylate (HY-B0190A), nitazoxanide (HY-B0217), ombitasvir (HY-13997), oseltamivir (HY-13318), penciclovir (HY-17424), perindopril salt (HY-B0130A), raltegravir (HY-10353), remdesivir (GS-5734, HY-104077), rilpivirine (HY-10574), ritonavir (HY-90001), simeprevir (HY-10241), sofosbuvir (HY-15005), and β-d-N4-hydroxycytidine (HY-125033) were purchased from MedChemExpress (Monmouth Junction, NJ, United States). Amodiaquin dihydrochloride dihydrate (A2799), artesunate (A3731), chloroquine phosphate (PHR1258), mefloquine hydrochloride (PHR1705), peramivir trihydrate (SML2486), ribavirin (R9644), teicoplanin (T0578), and toremifene citrate salt (T7204) were purchased from Sigma-Aldrich (St, Louis, Missouri, United States). Favipiravir (T-705) was provided by the Toyama Chemical Co., Ltd. (Japan). The drug solutions were filtered through a 0.22 μM membrane and were stored at −80°C.

Bang M.S., Kim C.M., Kim D.M, & Yun N.R. (2022). Effective Drugs Against Severe Fever With Thrombocytopenia Syndrome Virus in an in vitro Model. Frontiers in Medicine, 9, 839215.

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Physicochemical Characterization of Diverse Compounds

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In all, 18 compounds with different physiochemical properties were selected for this study. Antipyrine, ganciclovir, ofloxacin, 4‐aminoAntipyrine, lamotrigine, theophylline, citalopram, digoxin, quinidine, pemetrexed, atenolol, carbamazepine, propranolol, and diltiazem were purchased from MedChemExpress (Monmouth Junction). Acetaminophen was purchased from Sigma‐Aldrich. Fexofenadine and sumatriptan were purchased from Tokyo Chemical Industry. Cimetidine was purchased from National Institute for the Control of Pharmaceutical and Biological Products. All the other chemicals were of the HPLC grade or better.

Chen C., Zhou H., Guan C., Zhang H., Li Y., Jiang X., Dong Z., Tao Y., Du J., Wang S., Zhang T., Du N., Guo J., Wu Y., Song Z., Luan H., Wang Y., Du H., Zhang S., Li C., Chang H, & Wang T. (2020). Applicability of free drug hypothesis to drugs with good membrane permeability that are not efflux transporter substrates: A microdialysis study in rats. Pharmacology Research & Perspectives, 8(2), e00575.

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