Anti human cd45ra efluor 450

Cell surface and intracellular cytokine staining were performed as previously described (29 (link)). Monocyte phenotypes were analyzed by staining with anti-CD14-FITC (eBioscience), anti-CD16-PE (eBioscience), and anti-HLA-DR-eFluor® 450 (eBioscience) antibodies. For the intracellular staining of Foxp3⁺Helios⁺ Treg cells, we initially stained the cells for surface markers using the following antibodies: anti-CD4-PerCP-cyanine 5.5 (eBioscience), anti-human CD45RO-APC-eFluor® 780 (eBioscience), anti-human CD45RA-eFluor® 450 (eBioscience), and anti-human CD25-PE-Cy7 (eBioscience Inc., San Diego, CA). Cells were then fixed and permeabilized with the Foxp3 transcription factor staining buffer set, according to the manufacturer's instructions (eBioscience Inc., San Diego, CA), and the cells were then incubated with anti-Foxp3-PE (eBioscience) and anti-Helios APC (eBioscience Inc. San Diego CA) antibodies.

Cell surface and intracellular cytokine staining were performed as previously described [20 (link)]. Monocyte phenotypes and Foxp3⁺Helios⁺ Treg cells were stained with the following antibodies: anti-CD14-FITC (eBioscience), anti-CD16-PE (eBioscience), anti-HLA-DR-eFluor® 450 (eBioscience), anti-human CD279 (PD-1)-PerCP-eFluor® 710 (eBioscience); anti-CD4-PE-Cy7 (eBioscience), anti-xhuman CD45RO-APC-eFluor® 780 (eBioscience), anti-human CD45RA-eFluor® 450 (eBioscience), and anti-human CD25-PE-Cy7 (eBioscience) anti-Foxp3-PE (eBioscience), and anti-Helios APC (eBioscience Inc. San Diego CA).