Livers were fixed in 4% PFA solution for 2 hours at room temperature and incubated in 30% sucrose solution for 24 hours. After fixation, livers were embedded in Tissue-Tek O.C.T. Compound (Sakura Finetek) on dry ice and cut into 10 μm sections using a cryostat (Leica). Liver sections were fixed using 4% PFA for 10 min and incubated in block solution (10% BSA in PBS) for 1 hour. Sections were permeabilized using 0.2% Triton-X 100 for 30 min and incubated in primary antibodies for 24 hours at 4 °C (Rat anti-mouse CD31 (Biolegend), rat anti-mouse CD68 (Biolegend), rabbit anti-mouse desmin (Cell Signaling)). After 24 hours, sections were washed (PBS containing 2% BSA) 3 times and incubated in secondary antibodies for 30 min (goat anti-rat secondary antibody, Alexa Fluor 488 conjugate (Thermo Fischer Scientific)). Coverslips were mounted using VECTASHIELD anti-fade mounting medium with DAPI (Vector Labs).
Rabbit anti mouse desmin
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-mouse desmin is a primary antibody that recognizes the intermediate filament protein desmin. Desmin is a structural protein found in skeletal, cardiac, and smooth muscle cells. This antibody can be used to detect and localize desmin in various cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Rat anti mouse cd68, by BioLegend (1 mentions)
Rat anti mouse cd31, by BioLegend (1 mentions)
Alexa fluor 488 conjugate, by Thermo Fisher Scientific (1 mentions)
Tissue tek oct compound, by Sakura Finetek (1 mentions)
Vectashield antifade mounting medium with dapi, by Vector Laboratories (1 mentions)
4 6 diamidino 2 phenylindole dapi, by Abcam (1 mentions)
594 conjugated secondary antibodies, by Abcam (1 mentions)
Alexa fluor 488, by Abcam (1 mentions)
Tcs sp8, by Leica camera (1 mentions)
2 protocols using rabbit anti mouse desmin
1
Cryosectioning and Immunostaining of Murine Livers
2
Immunofluorescence Imaging of Liver Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NF-κB nuclear translocation and HBeAg cellular localization were detected with monoclonal antibody to P65, and GST tag. For NF-κB nuclear translocation, Alexa Fluor® 488 or 594-conjugated secondary antibodies (Abcam, Cambridge, UK) were incubated for 2 h. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, Abcam, Cambridge, UK). Double immunofluorescence staining of α-SMA and F4/80 was conducted on liver cryosections and incubated overnight with primary rat anti-mouse F4/80 (Abcam, Cambridge, UK) and rabbit anti-mouse-α-SMA (Cell-Signaling Technology, Boston, USA) and then with secondary antibodies and DAPI. Immunolocalization of Desmin and ki67 was performed on liver cryosections and incubated with primary rat anti-mouse ki67 (Thermo Fisher Scientific, Waltham, MA) and rabbit anti-mouse-Desmin (Cell-Signaling Technology, Boston, USA). Images were captured using a fluorescence microscope (Olympus BX63, Tokyo, Japan) or confocal fluorescence microscopy (Leica TCS SP8).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!