Magnesium sulfate

Diethoxydimethylsilane (1), diethoxymethylvinylsilane (2), and diethoxymethylsilane (3) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) Tri(ethylene glycol) divinyl ether (TEGDE), platinum (0)-1,3-divinyl-1,1,3,3,-tetramethyldisiloxane complex solution (0.05 mol % of platinum), and 37% hydrochloric solution in water were purchased from Sigma-Aldrich, Inc. (St. Louis, USA) Ethyl acetate (EA) and magnesium sulfate were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). All reagents were used as received.

Acetonitrile, 2-propanol, ampicillin, tributyrin, p-nitrophenyl (p-NP) acetate (C₂), p-NP butyrate (C₄), p-NP caprylate (C₈), lithium chloride, nickel chloride, sodium dodecyl sulfate, dithiothreitol, 2-mercaptoethanol, Triton X-100 and phenylmethylsulfonyl fluoride were purchased from Sigma Aldrich Co. (USA). Dimethyl sulfoxide, cobalt chloride hexahydrate, magnesium sulfate, potassium chloride, calcium chloride, sodium chloride, zinc chloride, chloroform, cyclohexane, n-hexane and acetone were purchased from Junsei Co. (Japan). p-NP caproate (C₆) was purchased from Tokyo Chemical Industry Co. (Japan). Methanol and ethanol were purchased from Merck Chemical Co. (Germany), and β-D-thiogalactopyranoside was purchased from Duchefa Biochemie B.V. Co. (The Netherlands). Copper sulfate was purchased from Kanto Chemical Co. (Japan). X-gal was purchased from Promega (USA). Ni-NTA agarose was purchased from Qiagen (Germany). We were provided with a lipolytic bacterial strain (No. 39-G1) isolated from the Arctic Beaufort Sea (70-56-1581 N, 136-25-1178 W) by Korea Polar Research Institute.

Sodium chloride (NaCl), lysozyme, monosodium phosphate (NaH₂PO₄), imidazole, glycerol, reduced glutathione, oxidized glutathione, methanol, dimethyl sulfoxide (DMSO), trifluoroacetic acid (TFA), tert-butyl acetate, perchloric acid (HClO₄), hydrochloric acid (HCl), sodium carbonate, ethyl acetate, sodium sulfate, hexane, sodium hydrogen carbonate (NaHCO₃), acetone, triphenyl phosphine (Ph₃P), anhydrous dichloromethane (CH₂Cl₂), and anhydrous tetrahydrofuran (THF) were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). 3-Bromo-tyrosine, 3-hydroxymethyl-2-methylbiphenyl, and diisopropyl azodicarboxylate (DIAD; 40% in toluene, approximately 1.9 mol L⁻¹) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Magnesium sulfate and CH₂Cl₂ were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). Deuterochloroform (CDCl₃) was purchased from Isotec, Inc. (Miamisburg, OH, USA), and N-[(9H-fluoren-9-ylmethoxy) carbonyloxy] succinimide (Fmoc-Osu) was purchased from Watanabe Chemical Industries, Ltd. (Hiroshima, Japan).