Micropublisher 5.0 rtv camera

After follow-up DSA was performed, the animals were then euthanized by using a lethal injection of pentobarbital. Median sternotomy and pericardiotomy were performed. Access to the left ventricle was obtained by direct puncture with a 20-gauge catheter. A small cut was made at the right atrial appendage. With pressure pump, heparinized saline (100U/ml) was continuously infused into the catheter, until the effluent from the right appendage was light pink. Then the animals were perfusion-fixed through the 20G catheter with 10% buffered formalin for 10 min followed by flushing with heparinized saline for 5 minutes. The coiled aneurysm was then harvested and immersed into tris-buffered saline (TBS). Under a dissection microscope (Leica MZ 125), the parent artery was cut longitudinally to expose the neck orifice for gross inspection to evaluate the tissue growth at the neck; the sample was then photographed using the MicroPublisher 5.0 RTV camera attached to the dissection microscope. After photography, the sample was fixed 10% formalin for 2 hours for further whole tissue mount staining. The adjacent left common carotid artery (LCCA) was harvested for positive and negative control.

After follow-up DSA, animals were killed by lethal injection of pentobarbital. Median sternotomy and pericardiotomy were performed. The animals were then perfusion-fixed with 10% buffered formalin for 10 min followed by flushing with heparinized saline for 5 min. The coiled aneurysm was then harvested and immersed in Tris-buffered saline (TBS). Under a dissection microscope (Leica MZ 125), the parent artery was cut longitudinally to expose the neck orifice for gross inspection to evaluate tissue growth at the neck; the sample was then photographed using the MicroPublisher 5.0 RTV camera attached to the dissection microscope. After photography, the sample was fixed in 10% formalin for 2 hours for further whole tissue mount staining.