Antibodies used for evaluating splenocytes and TILs were: anti-CD3 mAb (clone 17A2, 100222); anti-CD4 mAb (clone GK1.5; 100406); anti-CD8 mAb (clone 53-6.7; 100712); anti-CD11b mAb (clone M1/70; 101228); anti-Ly6C mAb (clone HK1.4; 128006); anti-Ly6G mAb (clone 1A8; 127608); anti-CD16/CD32 mAb (clone 2.4G2; 101320) from Biolegend (San Diego; CA, USA), anti-CD44 mAb (clone IM7; 17-0441-82); anti-CD25 mAb (clone PC61.5; 45-0251-82) from (Thermo Fisher), anti-CD62L mAb (clone MEL-14; 553152; BD Biosciences; San Jose, CA, USA).
Anti ly6g mab clone 1a8
Manufactured by BioLegend
Sourced in United States
Anti-Ly6G mAb (clone 1A8) is a monoclonal antibody that specifically binds to the Ly6G antigen. Ly6G is a cell surface marker expressed on neutrophils.
Automatically generated - may contain errors
2 protocols using anti ly6g mab clone 1a8
1
Multiparametric Flow Cytometry of Immune Cells
2
Neutrophil depletion and recruitment assay
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To deplete neutrophils in vivo, 0.25 mg depleting anti-Ly6G mAb (clone 1A8; BioLegend, San Diego, CA, USA) were injected i.p. 24 h before CLP procedure. For CXCR2 inhibition studies, the CXCR2 antagonist SB225002 (10 mg/kg, Cayman Chemical, Ann Arbor, MI, USA) were injected i.p. 1 h before CLP surgery (15 (link)). For the in vivo recruiting experiments, neutrophils from WT or Wip1 KO were labeled with PKH or CFSE in vitro and were injected i.v. together (4 × 106: 4 × 106) into neutrophil-depleted WT-recipient mice after CLP induction. PKH or CFSE-labeled neutrophils from WT or Wip1 KO donors were quantified in recipient peritoneal cavity 3 h after CLP by flow cytometry (Aria II; BD).
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