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The Aph1a is a laboratory equipment product offered by Thermo Fisher Scientific. It serves as a core component in various scientific applications, but a detailed description of its function cannot be provided while maintaining an unbiased and factual approach. Further information may be available from the manufacturer.

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Lab products found in correlation

Pen 2, by Abcam (2 mentions) Dc protein assay kit, by Bio-Rad (1 mentions) Glur2, by Merck Group (1 mentions) Myc 9e10, by Santa Cruz Biotechnology (1 mentions) Cleaved notch1 val1744, by Cell Signaling Technology (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) α tubulin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Neuronal nuclei (neun), by Merck Group (1 mentions) β actin, by Merck Group (1 mentions)

2 protocols using aph1a

1

Western Blot Analysis of Gamma-Secretase Complex

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Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris, pH8.0, 150 nM NaCl, 0.1% vol/vol Nonidet P-40, and 0.5% wt/vol deoxycholic acid) containing protease inhibitor mixture. Protein concentration was determined by the DC Protein Assay Kit (Bio-Rad). Antibodies used for Western blot are as follows: PS1-NTF and Nct (from our laboratory), PS1-CTF (MAB5232; Millipore), Aph1a (38-3600; Invitrogen), Pen2 (18189; Abcam), APP (MABN10; Millipore), and HA (18181; Abcam).
Wong E., Liao G.P., Chang J.C., Xu P., Li Y.M, & Greengard P. (2019). GSAP modulates γ-secretase specificity by inducing conformational change in PS1. Proceedings of the National Academy of Sciences of the United States of America, 116(13), 6385-6390.
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2

Characterization of APP processing in HEK293 cells

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HEK293 cells and HEK293 cells stably expressing human APP Swedish mutations (HEK293Swe) were cultured in DMEM (Hyclone) supplemented with 10% FBS (Gibco) and 1% penicillin/streptomycin (Gibco), in the absence and presence of 400 µg/mL G418 (Sigma), respectively.
Primary cortical neurons from embryonic day 17 (E17) rat pups were maintained in neurobasal medium supplemented with B27 and 0.8mM glutamine.
Antibodies used in these experiments include: NeuN (mouse mAb, Millipore), Nicastrin (mouse mAb, Abcam), Aph-1a (Invitrogen), Pen-2 (Abcam), Cleaved Notch1 (Val1744, Cell signaling), Anti-Notch1 intracellular domain antibody (rabbit pAb, Abcam), FLAG (mAb, clone M2, Sigma), GluR1 (mAb, Chemicon), GluR2 (pAb, Millipore), NR1 (mAb, BD Biosciences), α-tubulin (mAb, Sigma), β-actin (mAb, Sigma), Myc (9E10, Santa Cruz Biotechnology), Aβ (6E10, Covance); The rabbit polyclonal antibodies against PS1 loop (Thinakaran et al., 1996 (link)) and SNX27 (Balana et al., 2011 (link)) were described previously; The mouse monoclonal antibody (clone 3D5) against BACE1 was described previously (Zhao et al., 2007 (link)); The rabbit polyclonal antibody 369 against the APP C-terminus (Xu et al., 1997 (link)), Anti-Nicastrin (Ru716) and Anti-PS1 NTF antibody (Ab14) was developed in our laboratory.
Wang X., Huang T., Zhao Y., Zheng Q., Thompson R.C., Bu G., Zhang Y.W., Hong W, & Xu H. (2014). Sorting Nexin 27 Regulates Aβ Production through Modulating γ-secretase Activity. Cell reports, 9(3), 1023-1033.
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