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Cd19 pb

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CD19-PB is a fluorochrome-conjugated antibody that binds to the CD19 cell surface antigen, which is expressed on B cells. This product is intended for use in flow cytometry applications to identify and quantify B cell populations.

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Lab products found in correlation

Cd14 pe, by Thermo Fisher Scientific (1 mentions) Cd4 pe cy5, by BioLegend (1 mentions) Cd11c pe cy7, by Thermo Fisher Scientific (1 mentions) Cd14 pe, by BioLegend (1 mentions) Cd56 fitc, by BioLegend (1 mentions) Cd3 fitc, by Thermo Fisher Scientific (1 mentions) Cd19 efluor450, by Thermo Fisher Scientific (1 mentions) Cd8 pe tr, by Thermo Fisher Scientific (1 mentions) Cd11c apc, by BioLegend (1 mentions) Cd56 apc, by BioLegend (1 mentions) Cd11b apc cy7, by Thermo Fisher Scientific (1 mentions) Cd11c fitc, by BioLegend (1 mentions) Cd3 pe cy7, by BioLegend (1 mentions) Cd45 apc cy7, by BioLegend (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Cd27 apc, by BioLegend (1 mentions) Cd38 pe, by BioLegend (1 mentions) Moflo astrios eq flow cytometer, by Beckman Coulter (1 mentions) Igm pe, by BD (1 mentions) Cd80 pe, by BioLegend (1 mentions)

3 protocols using cd19 pb

1

Adoptive transfer of irradiated OVA-splenocytes

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Splenocytes (SPC) were obtained from B6, Mb1 or μMT mouse recipients that were treated by the adoptive transfer of irra-OvaSPC. Cells were also collected from the draining lymph node (DLN) and non-draining lymph node (Non-DLN) and stained with the following antibodies: CD19-PB (6D5, Biolegend), IgM-PE (R6–60.2, BD Biosciences), IgD-Percp/Cy5.5 (11–26c.2a, Biolegend), CD21-APC (eBio4E3, eBioscience), CD23-PE/Cy7 (B3B4, Biolegend), CD80-PE (16–10A1, Biolegend), CD86-APC/Cy7 (GL-1, Biolegend), CD19-APC (6D5, Biolegend), Blimp1-PE (5E7, Biolegend), LAP-PE (TGF-β, TW7–16B4, Biolegend), CD365/Tim-1 PE (RMT1–4, Biolegend). Detection of antibodies was done in sera from B6 mice that were incubated with Ova+ SPC, and stained with CD19-PB, IgM-PE, and IgG-FITC (Poly4060, Biolegend). All results were analyzed using the FlowJo software (Treestar, Ashland, OR, USA).
Fu Q., Lee K.M., Huai G., Deng K., Agarwal D., Rickert C.G., Feeney N., Matheson R., Yang H., LeGuern C., Deng S, & Markmann J.F. (2021). Properties of Regulatory B Cells Regulating B Cell Targets. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 21(12), 3847-3857.
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2

Comprehensive Immune Cell Profiling

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Antibodies used in hu-PBMC staining; CD3-FITC, CD19-eFluor®450, CD56-PerCP-Cy5, CD14-PE, CD11b-APC-Cy7, and CD11c –PE-Cy7 (eBioscience). Live cells were determined using the Live/Dead Aqua Fixable Dead Stain Kit (Invitrogen). Antibodies used for humanized mouse experiments: CD11c-FITC, CD56-FITC, CD14-PE, CD4-PE-Cy5, CD3-PE-Cy7, CD123-PE-Cy5, CD19-PB, CD11c-APC, CD56-APC, CD45-APC-Cy7 (Biolegend); CD8-PE-TR, CD4-PE-TR, mouse CD45-Pacific Orange (Invitrogen). FACS buffer; 1×PBS (Gibco) + 2% FBS (Sigma). All data were collected using an LSRII (BD Biosciences) or CyAn™ (Beckman Coulter) flow cytometer and analyzed using FlowJo (Tree Star, Inc.).
Robbins G., Roberts R., Guo H., Reuter K., Shen T., Sempowski G., McKinnon K.P., Su L., DeSimone J, & Ting J. (2015). Analysis of Human Innate Immune Responses to PRINT Fabricated Nanoparticles with Cross Validation Using a Humanized Mouse Model. Nanomedicine : nanotechnology, biology, and medicine, 11(3), 589-599.
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3

Sorting COVID-19 Antibody-Producing Cells

Cited 1 time
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Flow cytometry analysis was performed as previously described.52 (link) PBMCs were isolated from blood of COVID-19 convalescent subjects using human lymphocyte separation medium. Cells were stained and mixed with fluorescent-labeled DNA-barcoded antigens and other fluorescent antibodies, and sorted by FACS with a MoFlo Astrios EQ Flow Cytometer (Beckman). The following antibodies were used to label the B cell subgroups: CD19-PB (BioLegend, Cat 302232), CD27-APC (BioLegend, Cat 302810), CD38-PE (BioLegend, Cat 303506). CD19+ CD27+ memory B cells and CD19+ CD27high CD38high plasma B cells were sorted for further scRNA-seq.
He B., Liu S., Wang Y., Xu M., Cai W., Liu J., Bai W., Ye S., Ma Y., Hu H., Meng H., Sun T., Li Y., Luo H., Shi M., Du X., Zhao W., Chen S., Yang J., Zhu H., Jie Y., Yang Y., Guo D., Wang Q., Liu Y., Yan H., Wang M, & Chen Y.Q. (2021). Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq. Signal Transduction and Targeted Therapy, 6, 195.
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