Rabbit p akt

Manufactured by Abcam

Rabbit p-Akt is a primary antibody that specifically recognizes the phosphorylated form of the Akt (Protein Kinase B) protein. Akt is a key regulator of cellular processes such as cell growth, proliferation, and survival. The phosphorylated form of Akt represents the active state of this protein.

Automatically generated - may contain errors

Lab products found in correlation

Bicinchoninic acid assay, by Thermo Fisher Scientific (4 mentions) Il 12, by Abcam (2 mentions) Rabbit p erk, by Cell Signaling Technology (2 mentions) Rabbit akt, by Abcam (2 mentions) Rabbit jnk, by Abcam (2 mentions) Rabbit p jnk, by Abcam (2 mentions) Rabbit il 10, by Abcam (2 mentions) Rabbit erk, by Cell Signaling Technology (2 mentions) Rabbit β tubulin, by Merck Group (2 mentions) Sds polyacrylamide gel electrophoresis, by Thermo Fisher Scientific (1 mentions) Sds page, by Thermo Fisher Scientific (1 mentions) Rabbit gapdh, by Proteintech (1 mentions) Chemidoc imaging system, by Bio-Rad (1 mentions) Rabbit psd95, by Abcam (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Beyotime (1 mentions) Hrp conjugated goat anti rabbit igg, by Proteintech (1 mentions)

3 protocols using rabbit p akt

Characterization of Macrophage and Smooth Muscle Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peritoneal macrophages, RAW 264.7 and hASMCs were homogenized in RIPA lysis buffer. Protein concentrations were determined through the Bicinchoninic Acid assay (Thermo Scientific) according to the manufacturer’s protocol. SDS–polyacrylamide gel electrophoresis (Invitrogen) was performed using 10 μg of protein per well. Western blot was performed with the following primary antibodies: rabbit Chi3l1 (dilution 1:1,000), rabbit Akt (1:500), rabbit p-Akt (1:500), rabbit JNK (1:3,000), rabbit p-JNK (1:3,000) and rabbit IL-10 (1:1,000; all obtained from Abcam), and IL-12 (1:1,000), rabbit ERK (1:3,000) and rabbit p-ERK (1:3,000; all obtained from Cell Signaling), and rabbit β-tubulin (1:1,000; obtained from Sigma-Aldrich; Supplementary Fig. 10).

Maegdefessel L., Spin J.M., Raaz U., Eken S.M., Toh R., Azuma J., Adam M., Nagakami F., Heymann H.M., Chernugobova E., Jin H., Roy J., Hultgren R., Caidahl K., Schrepfer S., Hamsten A., Eriksson P., McConnell M.V., Dalman R.L, & Tsao P.S. (2014). miR-24 limits aortic vascular inflammation and murine abdominal aneurysm development. Nature Communications, 5, 5214.

+ Open protocol

+ Expand

Protein Expression and Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peritoneal macrophages, RAW 264.7, and hASMCs were homogenized in RIPA lysis buffer. Protein concentrations were determined through the Bicinchoninic Acid assay (ThermoScientific), according to the manufacturer's protocol. SDS-PAGE (Invitrogen) was performed using 10µg of protein per well. Western blot was performed with the following primary antibodies: rabbit Chi3l1 (dilution 1:1000), rabbit Akt (1:500), rabbit p-Akt (1:500), rabbit JNK (1:3000), rabbit p-JNK (1:3000), rabbit IL-10 (1:1000; all from Abcam) and IL-12 (1:1000), rabbit ERK (1:3000), rabbit p-ERK (1:3000; all from Cell Signaling) and rabbit β-tubulin (1: 1000; both Sigma Aldrich; Supplementary Fig. 10).

+ Open protocol

+ Expand

Protein Expression Analysis in Rat PFCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The PFCs of rats in each group were separated and weighed with an electronic balance. The tissue was cut into pieces with scissors, washed 3 times with saline, and dissolved in a mixture of RIPA buffer and 2 mM PMSF (Beyotime Biotech, Shanghai, China). The protein concentration was determined using the BCA protein assay kit (Beyotime). Then the protein was diluted with PBS and loading buffer and boiled for 5 min. 30 μg total protein in each group was electrophoretically separated by 8% or 10% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gels, and incubated at 4 °C overnight with primary antibodies as follows: rabbit MeCP2 (1:5000, Abcam), rabbit BDNF (1:1000, Abcam), rabbit TrkB (1:1000, Abcam), rabbit PSD95 (1:1000, Abcam), rabbit ERK (1:2000, Abcam), rabbit p-ERK (1:1000, Abcam), rabbit Akt (1:2000, Abcam), rabbit p-Akt (1:1000, Abcam) and rabbit GAPDH (1:8000; Proteintech). The membrane was washed three times in TBST, and then incubated with HRP- conjugated goat anti-rabbit IgG (1:10000; Proteintech) at 37 °C for another 1 h, followed by ECL detection and imaging using a Bio-Rad ChemiDoc imaging system (Bio-Rad).

Tong L., Li M.D., Nie P.Y., Chen Y., Chen Y.L, & Ji L.L. (2021). miR-132 downregulation alleviates behavioral impairment of rats exposed to single prolonged stress, reduces the level of apoptosis in PFC, and upregulates the expression of MeCP2 and BDNF. Neurobiology of Stress, 14, 100311.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!