Biotin conjugated goat anti gfp

Manufactured by Rockland Immunochemicals

Biotin-conjugated goat anti-GFP is a secondary antibody used to detect and bind to green fluorescent protein (GFP) in various biological applications. The biotin molecule attached to the antibody can be further utilized for signal amplification or detection purposes.

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2 protocols using biotin conjugated goat anti gfp

Antibody Staining for EHD and Tubulin

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The following antibodies were used (also see Table 1): Rabbit anti‐EHD1 (Abcam, ab109311), rabbit anti‐EHD4,²⁹ Rabbit anti‐acetyl‐α‐tubulin (Lys40) (D20G3) (Cell Signaling, 5335), mouse anti‐acetylated tubulin (Sigma‐Aldrich, T7451), rabbit anti‐CP110 (ProteinTech, 12780‐1‐AP), rabbit anti‐ARL13B (ProteinTech, 17711‐1‐AP), mouse anti‐GFP (Roche, 11814460001), mouse anti‐pan actin (Novus, NB600‐535), mouse anti‐GAPDH‐HRP (ProteinTech, HRP‐60004), donkey anti‐mouse‐HRP (Jackson, 715‐035‐151), mouse anti‐rabbit IgG light chain‐HRP (Jackson, 211‐032‐171), DAPI (4′,6‐diamidino‐2‐phenylindole, dihydrochloride) (Molecular Probes, D1306), biotin‐conjugated goat anti‐GFP (Rockland, 600‐106‐215), Alexa‐fluor 488‐conjugated streptavidin (Molecular Probes, S11223), Alexa‐fluor 488‐conjugated goat anti‐mouse (Molecular Probes, A11029), Alexa‐fluor 568‐conjugated goat anti‐rabbit (Molecular Probes, A11036), Alexa‐fluor 568‐conjugated goat anti‐mouse (Molecular Probes, A11031), Alexa‐fluor 633‐conjugated goat anti‐rabbit (Molecular Probes, A21070).

Jones T., Naslavsky N, & Caplan S. (2022). Differential requirements for the Eps15 homology domain proteins EHD4 and EHD2 in the regulation of mammalian ciliogenesis. Traffic (Copenhagen, Denmark), 23(7), 360-373.

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Comprehensive Antibody Evaluation for LC3 Detection

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The following commercially available antibodies against (human) LC3s were tested: mouse monoclonal anti-LC3B, clone LC3-1703 (Cosmo Bio, CAC-CTB-LC3-2-IC); rabbit anti-LC3A/B (MBL, M152-3); rabbit polyclonal and monoclonal anti-total LC3A/B and rabbit polyclonal anti-LC3B from Cell Signaling Technology (4108, 12741 and 2775, respectively); rabbit polyclonal anti-total LC3A/B from Sigma (L8918); rabbit monoclonal anti-LC3A from Millipore (clone EP1528Y, MABC177); rabbit anti-LC3B (Novus Biologicals, NB600-1384) and mouse anti-LC3B, clone 5F10 (Nanotools, 0231–100/LC3-5 F10). In addition, we used guinea pig anti-SQSTM1/p62 (C terminus) from Progen (GP62-C), rabbit anti-mouse IgG, biotin-conjugated goat anti-GFP and rabbit anti-biotin antibodies from Rockland (610–4120, 600–106-215 and 100–4198, respectively), rabbit anti-GFP, Alexa Fluor 488 PLUS-conjugated goat anti-mouse and donkey anti-rabbit IgG from Thermo Fisher Scientific (A6455, A32723 and A-21206, respectively). For IF on whole cells, we used secondary antibodies Alexa Fluor 568-conjugated rabbit anti-mouse (Life Technologies, A11061) and Alexa Fluor 568-conjugated donkey anti-rabbit (Life Technologies, A10042) IgG. Rabbit anti-LAMP1 [61 (link)] was a gift from M Fukuda.

De Mazière A., van der Beek J., van Dijk S., de Heus C., Reggiori F., Koike M, & Klumperman J. (2022). An optimized protocol for immuno-electron microscopy of endogenous LC3. Autophagy, 18(12), 3004-3022.

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