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2 protocols using anti mta2

1

Comprehensive Chromatin Remodeling Antibody Panel

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The sources of the antibodies were anti-FLAG, anti-HDAC1 anti-HDAC2, anti-RbAp46/48, anti-Fibronectin, anti-Vimentin and anti-β-actin (Sigma–Aldrich); anti-DDB1, anti-MTA1, anti-SIN3A, anti-SAP180 and anti-SAP30 (Santa Cruz Biotechnology); anti-PRMT5, anti-ROC1, anti-LSD1, anti-DNMT3B, anti-HDAC5 and anti-MTA2 (Abcam); anti-EED, anti-MBD2/3 and anti-MTA3 (Millipore); anti-SUZ12 (Cell Signaling Technology); anti-WDR77 (also known as MEP50, Bethyl); anti-MTA2, anti-E-cadherin, anti-α-catenin, anti-γ-catenin, anti-N-cadherin and anti-EZH2 (BD Bioscience); anti-ING4 (Genetex), anti-SETD2 (Proteintech). Dynabeads Protein G was obtained from Invitrogen by Thermo Fisher Scientific, and protease inhibitor mixture cocktail was from Roche Applied Science. Glutathione-Sepharose 4B beads were from GE Healthcare Bio-Sciences. Short hairpin RNAs (shRNAs) were obtained from GenePharma Co Ltd (Shanghai, China).
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2

Antibody Sources and Inhibitors for Protein Analysis

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Anti-FLAG antibody was purchased from Sigma–Aldrich (Cat. F1804). Anti-SMYD3 (Cat. ab187149), anti-DDB1(Cat. ab109027), anti-METTL3 (Cat. ab195352), anti-HDAC1 (Cat. ab7028), anti-HDAC2 (Cat. ab7029), anti-MTA2 (Cat. ab8106), anti-Histone H4 (Cat. ab177840), and anti-MBD3 (Cat. ab157464) were purchased from Abcam. Anti-CUL4B (Cat. 12916–1-AP), anti-LDHA (Cat. 19987–1-AP), anti-METTL1 (Cat. 14994–1-AP), anti-IGFBP4 (Cat. 18500–1-AP), and anti-β-actin (Cat. 66009–1-lg) were purchased from Proteintech. Anti-MAT1 (Cat. D17G10) and anti-RBBP4 (Cat. 4633-S) were purchased from Cell Signaling Technology. Anti-SIRT1 antibody was purchased from Millipore (Cat. 07–131). anti-Histone H4ac was purchased from ACTIVE MOTIF (Cat. 39243). Glutathione-Sepharose 4B beads were purchased from GE Healthcare Bio-Sciences (Cat. 17–0756-01). Dynabeads Protein G was obtained from Invitrogen by Thermo Fisher Scientific (Cat. 10004D), and the protease inhibitor mixture cocktail was from Roche Applied Science (Cat. 04 693 116 001). Inhibitors of SMYD3, including BCI-121 (Cat. HY-21972) and EPZ031686 (Cat. HY19324), were purchased from MedChemExpress (MCE). siRNAs were purchased from RiboBio (RiboBio Co. Ltd., Guangzhou, Guangdong). siRNA sequences are listed in Additional file 1: Table S1.
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