Liver hydrolysate

N. lovaniensis trophozoites (ATCC #30569) were cultivated at 36 °C in different media types according to Burri et al. [15 (link)] using Nunclon™ Δ Surface cell culture flasks (Thermo Fisher Scientific, Allschwil, Switzerland). Nelson’s Medium contains 0.1% (w/v) Liver Hydrolysate (Sigma, Buchs, Switzerland), 0.1% (w/v) D-(+)-glucose (Sigma), and 10% (v/v) fetal calf serum in Page’s amoeba saline (2 mM NaCl, 16 μM MgSO₄, 27.2 μM CaCl₂, 1 mM Na₂HPO₄, 1 mM KH₂PO₄). PYNFH medium consists of 1% (w/v) Bacto peptone (BD Biosciences), 1% (w/v) yeast extract (BD Biosciences), 0.1% (w/v) yeast ribonucleic acid (Sigma), 15 mg folic acid (Sigma) and 1 mg haemin (Sigma) supplemented with 10% (v/v) fetal calf serum in 133 mM KH₂PO₄, 176.1 mM Na₂HPO₄. As a third media type, PYNFH medium was supplemented with 0.1% (w/v) Liver Hydrolysate (Sigma, Buchs, Switzerland).