Hplc grade tfa

Manufactured by Thermo Fisher Scientific

HPLC grade TFA is a highly pure trifluoroacetic acid solution designed for use in high-performance liquid chromatography (HPLC) applications. It serves as a common ion-pairing agent and mobile phase component in HPLC separations.

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Lab products found in correlation

Anhydrous solvents, by Merck Group (2 mentions) Agilent 6210 lc ms, by Agilent Technologies (2 mentions) Agilent 6120 single quadrupole 1220 lcms, by Agilent Technologies (2 mentions) Silica gel 60 f254 plates, by Thermo Fisher Scientific (2 mentions) Burdick and jackson hplc grade solvents, by Avantor (2 mentions) Zorbax sb c18 column, by Agilent Technologies (2 mentions) Superdex 200 5 150 gl column, by GE Healthcare (1 mentions) Hplc grade h2o, by Thermo Fisher Scientific (1 mentions) Optilab dsp, by Wyatt Technology (1 mentions) 500 mhz spectrometer, by Bruker (1 mentions) Mercury 400 mhz spectrometer, by Agilent Technologies (1 mentions)

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HPLC grade (157 citations) HPLC grade trifluoroacetic acid (TFA) (2 citations)

3 protocols using hplc grade tfa

Analytical-scale HPLC and SEC-MALS analysis

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Analytical-scale HPLC was performed with an Agilent 1200 series system on octadecyl carbon chain (C18)–bonded silica columns (5-μm pore size, 4.6 × 250 mm, 218TP54, Vydac). The solvent system was HPLC-grade H₂O (W/0106/PB17, Fisher Scientific) and HPLC-grade acetonitrile (ACN; A/0627/PB17, Fisher Scientific). Buffer A consisted of HPLC-grade H₂O containing 0.1% (v/v) HPLC-grade TFA (T/3258/04, Fisher Scientific), and buffer B consisted of 80% (v/v) ACN in HPLC-grade H₂O containing 0.1% (v/v) TFA.
Mass spectrometry analysis was performed on the Agilent Series 1100 LC-MS system in positive mode of ESI. The solvent system was as follows. Buffer A consisted of LC-MS grade H₂O (W/0112/17, Fisher Scientific) containing 0.1% (v/v) formic acid (06440, Fluka), and buffer B consisted of 80% (v/v) ACN in MS-LC–grade H₂O containing 0.1% (v/v) formic acid. Data analysis was performed with LC/MSD ChemStation software.
SEC-MALS was performed on a Superdex 200 5/150 GL column (GE Healthcare) in SEC-MALS buffer (50 mmol/liter Tris-HCl (pH 7.4), 5 mm MgCl₂, 1 mmol/liter CaCl₂, 100 mm NaCl, and 100 mmol/liter tris(2-carboxyethyl)phosphine). Recombinant troponins were extensively dialyzed against SEC-MALS buffer before experiments. Light scattering and refractive index were measured on a Mini DAWN and OPTILAB DSP (Wyatt Technology, UK), respectively. Data were analyzed with custom-written software.

Sevrieva I.R., Brandmeier B., Ponnam S., Gautel M., Irving M., Campbell K.S., Sun Y.B, & Kampourakis T. (2020). Cardiac myosin regulatory light chain kinase modulates cardiac contractility by phosphorylating both myosin regulatory light chain and troponin I. The Journal of Biological Chemistry, 295(14), 4398-4410.

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Spectroscopic Characterization of Chemical Compounds

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All reagents were purchased from commercial suppliers and used without further purification. ¹H NMR spectra were recorded on Bruker 500 MHz spectrometer with CDCl₃, CD₃OD or DMSO-d₆ as the solvent. ¹³C NMR spectra were recorded at 125 MHz. All coupling constants are measured in Hertz (Hz) and the chemical shifts (δ_H and δ_C) are quoted in parts per million (ppm) relative to TMS (δ 0), which was used as the internal standard. High resolution mass spectroscopy was carried out on an Agilent 6210 LC/MS (ESI-TOF). HPLC-MS analysis was performed using Agilent 6120 single quadrupole 1220 LCMS equipped with Zorbax SB-C18 column (4.6 x 50 mm, 1.8 micron). The purity of final compounds that underwent biological assessment were >95% as measured by HPLC-MS. Thin layer chromatography was performed using silica gel 60 F254 plates (Fisher), with observation under UV when necessary. Anhydrous solvents (acetonitrile, dimethylformamide, ethanol, isopropanol, methanol and tetrahydrofuran) were used as purchased from Aldrich. Burdick and Jackson HPLC grade solvents (methanol, acetonitrile and water) were purchased from VWR for HPLC and high-resolution mass analysis. HPLC grade TFA was purchased from Fisher. Compound synthesis and characterization are detailed in the Supporting Information.

Davis R.R., Li B., Yun S.Y., Chan A., Nareddy P., Gunawan S., Ayaz M., Lawrence H.R., Reuther G.W., Lawrence N.J, & Schönbrunn E. (2021). Structural insights into JAK2 inhibition by ruxolitinib, fedratinib, and derivatives thereof. Journal of medicinal chemistry, 64(4), 2228-2241.

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Synthesis and Characterization of (Rac)-i-Talazoparib

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All reagents were purchased from commercial suppliers and used without further purification. ¹H NMR spectra were recorded on an Agilent-Varian Mercury 400 MHz spectrometer with CDCl₃, CD₃OD or DMSO-d₆ as the solvent. ¹³C NMR spectra are recorded at 100 MHz. All coupling constants are measured in Hertz (Hz) and the chemical shifts (δ_H and δ_C) are quoted in parts per million (ppm) relative to TMS (δ 0), which was used as the internal standard. High resolution mass spectroscopy was carried out on an Agilent 6210 LC/MS (ESI-TOF). HPLC-MS analysis was performed using Agilent 6120 single quadrupole 1220 LCMS equipped with Zorbax SB-C18 column (4.6 × 50 mm, 1.8 micron). The purity of final compounds that underwent biological assessment were >95% as measured by HPLC-MS. Thin layer chromatography was performed using silica gel 60 F254 plates (Fisher), with observation under UV when necessary. Anhydrous solvents (acetonitrile, dimethylformamide, ethanol, isopropanol, methanol and tetrahydrofuran) were used as purchased from Aldrich. Burdick and Jackson HPLC grade solvents (methanol, acetonitrile and water) were purchased from VWR for HPLC and high resolution mass analysis. HPLC grade TFA was purchased from Fisher.
The compound 2, (Rac)-i-Talazoparib, was synthesized using similar methods decribed for preparation of talazoparib (1) (WANG ET AL., 2011 ).

Palve V., Knezevic C.E., Bejan D.S., Luo Y., Li X., Novakova S., Welsh E.A., Fang B., Kinose F., Haura E.B., Monteiro A.N., Koomen J.M., Cohen M.S., Lawrence H.R, & Rix U. (2021). The non-canonical target PARP16 contributes to polypharmacology of the PARP inhibitor talazoparib and its synergy with WEE1 inhibitors. Cell chemical biology, 29(2), 202-214.e7.

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