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Ac187

Manufactured by Bio-Techne
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Sourced in United Kingdom

AC187 is a laboratory instrument designed for protein analysis. It is capable of Western blotting, a widely used technique in molecular biology and biochemistry to detect and quantify specific proteins in a sample. The core function of AC187 is to facilitate this analytical process, but detailed specifications are not available for an unbiased, factual description.

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Lab products found in correlation

Bromodeoxyuridine (brdu), by Merck Group (1 mentions) Amylin, by Bachem (1 mentions) Blebbistatin blebb, by Bio-Techne (1 mentions) Sphingosine 1 phosphate (s1p), by Merck Group (1 mentions) Exenatide, by Bachem (1 mentions) Cck 8, by Bachem (1 mentions)

7 protocols using ac187

1

Alcohol-Seeking Behavior Modulation

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sCT (Tocris Bioscience, Bristol, United Kingdom) was diluted in vehicle (0.9% sodium chloride solution), and administered intraperitoneally (IP) at the doses of 1 or 5 μg/kg always 30 min prior to alcohol presentation. AC187 (Tocris Bioscience, Bristol, United Kingdom) was diluted in vehicle (0.9% sodium chloride solution) and administered at a dose of 250 μg/kg (IP), 5 min prior to alcohol presentation, in order to compensate for the drug’s short half-life and probable short bioavailability [3 (link), 7 (link)].
Kalafateli A.L., Vallöf D., Colombo G., Lorrai I., Maccioni P, & Jerlhag E. (2019). An amylin analogue attenuates alcohol-related behaviours in various animal models of alcohol use disorder. Neuropsychopharmacology, 44(6), 1093-1102.
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2

Preparation of Amylin, AC187, and BrdU

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Amylin (Bachem AG, Bubendorf, Switzerland; catalog number: H-9475.1000) was reconstituted in sterile 0.9% NaCl, AC187 (TOCRIS Bioscience, Bristol, UK; catalog number: 3419) was reconstituted in sterile double-distillated water. BrdU (B5002, Sigma Aldrich, Buchs, Switzerland) was dissolved in sterile double-distillated water and heated to 40–50 °C.
Liberini C.G., Borner T., Boyle C.N, & Lutz T.A. (2016). The satiating hormone amylin enhances neurogenesis in the area postrema of adult rats. Molecular Metabolism, 5(10), 834-843.
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3

Calcitonin Receptor Antagonist and Pramlintide Effects on Tumor Growth

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2×105 cells were plated in duplicate in a 6-well dish. Twelve hours later, cells were treated with Calcitonin receptor antagonist (1 nM)(AC187, Tocris Bioscience) for a period of 48 hours with or without simultaneous pramlintide treatment. Similarly, a chronic dose of Calcitonin receptor antagonist (1 nM/gram of tumour weight) was administered through non-invasive intra-thymic injections with one injection every week for a period of three weeks with or without simultaneous pramlintide treatment. Tumour volume was monitored and measured weekly by MRI.
Venkatanarayan A., Raulji P., Norton W., Chakravarti D., Coarfa C., Su X., Sandur S.K., Ramirez M.S., Lee J., Kingsley C.V., Sananikone E.F., Rajapakshe K., Naff K., Parker-Thornburg J., Bankson J.A., Tsai K.Y., Gunaratne P.H, & Flores E.R. (2014). IAPP driven metabolic reprogramming induces regression of p53 - deficient tumours in vivo. Nature, 517(7536), 626-630.
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4

Calcitonin Receptor Antagonist and Pramlintide Effects on Tumor Growth

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2×105 cells were plated in duplicate in a 6-well dish. Twelve hours later, cells were treated with Calcitonin receptor antagonist (1 nM)(AC187, Tocris Bioscience) for a period of 48 hours with or without simultaneous pramlintide treatment. Similarly, a chronic dose of Calcitonin receptor antagonist (1 nM/gram of tumour weight) was administered through non-invasive intra-thymic injections with one injection every week for a period of three weeks with or without simultaneous pramlintide treatment. Tumour volume was monitored and measured weekly by MRI.
Venkatanarayan A., Raulji P., Norton W., Chakravarti D., Coarfa C., Su X., Sandur S.K., Ramirez M.S., Lee J., Kingsley C.V., Sananikone E.F., Rajapakshe K., Naff K., Parker-Thornburg J., Bankson J.A., Tsai K.Y., Gunaratne P.H, & Flores E.R. (2014). IAPP driven metabolic reprogramming induces regression of p53 - deficient tumours in vivo. Nature, 517(7536), 626-630.
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5

Preparation and Dosing of Neuroactive Compounds

Cited 1 time
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Salmon calcitonin (sCT; Bachem) was dissolved in artificial cerebrospinal fluid (aCSF; Harvard Apparatus) for central injections and in sterile 0.9% saline for peripheral injections. Angiotensin II (AngII; Bachem) and AC187 (Tocris) were dissolved in aCSF for central injections. Behaviorally relevant doses were selected from the literature (Daniels et al., 2005 (link); Mietlicki et al., 2009 (link); Mietlicki-Baase et al., 2015b (link); Mietlicki-Baase et al., 2013 (link)).
Mietlicki-Baase E.G., McGrath L.E., Koch-Laskowski K., Krawczyk J., Reiner D.J., Pham T., Nguyen C.T., Turner C.A., Olivos D.R., Wimmer M.E., Schmidt H.D, & Hayes M.R. (2017). Amylin receptor activation in the ventral tegmental area reduces motivated ingestive behavior. Neuropharmacology, 123, 67-79.
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6

Preparing IAPP Oligomers for Research

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IAPP oligomers were prepared based on a previously published protocol [16 (link)], which yields IAPP bands between 4 and 25 kDa on a western blot [11 (link)]. Briefly, lyophilized synthetic human IAPP1-37 (AlexoTech AB #AI-452-10) was solubilized in 10 mM NaOH (pH 11). For adjusting the pH to 7, phosphate buffer was added to a final concentration of 100 µM. For oligomer formation, IAPP was incubated with agitation for 20 min at room temperature. Pramlintide acetate salt (Pram) (Sigma Aldrich #SML2523) Y27632 (Sigma Aldrich, #SCM075), and AC187 (Tocris, #3419) were resuspended in bi-distilled H2O. Sphingosine 1 phosphate (S1P) (Sigma Aldrich, #73914-1MG) was solubilized in NaOH 0.3 M to a stock concentration of 1 mM. Blebbistatin (Blebb) (Tocris, #1760) was resuspended in dimethyl sulfoxide to a concentration of 100 mM.
Nuñez-Diaz C., Pocevičiūtė D., Schultz N., Welinder C., Swärd K, & Wennström M. (2023). Contraction of human brain vascular pericytes in response to islet amyloid polypeptide is reversed by pramlintide. Molecular Brain, 16, 25.
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7

Exenatide and CCK-8 Analogues Protocol

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Exenatide and CCK-8 were purchased from Bachem. AC3174 (an Exenatide analogue), AC170222 (a CCKR1-selective agonist), and AC170236 (a CCKR2-selective agonist) were synthesised at Amylin Pharmaceuticals and provided via MedImmune/AstraZeneca through its acquisition of Amylin Pharmaceuticals as previously described [10 ]. AC187 was purchased from Tocris and used at 100 μg/kg.
Roth E., Benoit S., Quentin B., Lam B., Will S., Ma M., Heeley N., Darwish T., Shrestha Y., Gribble F., Reimann F., Pshenichnaya I., Yeo G., Baker D.J., Trevaskis J.L, & Blouet C. (2020). Behavioural and neurochemical mechanisms underpinning the feeding-suppressive effect of GLP-1/CCK combinatorial therapy. Molecular Metabolism, 43, 101118.
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