Qplus185 system

Imidazol formate, formic acid, acetonitrile, sulfatase, 2-propanol, 2,2-azinobis-(ethyl-2,3-dihydrobenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), 1,2-benzenedithiol, 3,3′-diindolylmethane (DIM), and indole-3-carbinol (I3C) were obtained from Sigma-Aldrich (Darmstadt, Germany). Glucotropaeolin (GTL) was from AppliChem (Darmstadt, Germany), indole-3-acetonitrile (I3ACN) from Merck (Darmstadt, Germany), and HPLC grade methanol from Chempur (Piekary Śląskie, Poland). Water was purified with a QPLUS185 system from Millipore (Burlington, MA, USA).

The following chemicals and reagents were purchased from Sigma-Aldrich (Taufkirchen, Germany): 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis-(ethyl-2,3-dihydrobenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sodium thiosulfate, thiazolyl blue tetrazolium bromide (MTT), phosphate-buffered saline (PBS), dimethyl sulfoxide (DMSO), oleuropein (purity ≥ 98.0%, CAS-No: 32619-42-4), echinacoside (purity ≥ 98.0%, CAS-No: 82854-37-3), rutin (≥94.0%, CAS-No: 207671-50-9), 2-propanol, diphenylamine, aniline, McCoys’ 5A medium (modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture), fetal bovine serum (FBS, non-USA origin, sterile-filtered, suitable for cell culture), penicillin–streptomycin (solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture), formic acid, α-amylase from porcine pancreas (Type VI, 5 U/mg) and acarbose. Acetonitrile was from Merck (Darmstadt, Germany). Ethanol, methanol, sulfuric acid, phosphate acid, boric acid, 1-butanol, starch soluble and sodium persulfate were from POCH (Gliwice, Poland). Water was purified with a QPLUS185 system from Millipore (Burlington, MA, USA).