Mouse actb

Manufactured by Thermo Fisher Scientific

Mouse ACTB is a gene that encodes the protein beta-actin, a cytoskeletal protein involved in cell motility, structure, and integrity. It is commonly used as a reference gene for normalizing gene expression levels in various molecular biology techniques.

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Lab products found in correlation

Random hexamer, by Thermo Fisher Scientific (1 mentions) Allprep dna rna kit, by Qiagen (1 mentions) Mx3005p qpcr system, by Agilent Technologies (1 mentions) Mini beadbeater 1, by Biospec (1 mentions) Mirvana mirna kit, by Thermo Fisher Scientific (1 mentions) Abi prism 7500, by Thermo Fisher Scientific (1 mentions) Taqman reverse transcription kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-ACTB mouse monoclonal antibody Mouse ACTB mix VIC‐labeled Mouse ACTB Mouse Actb (Mm02619580_g1;

2 protocols using mouse actb

Trigeminal Ganglia Gene Expression Analysis

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The trigeminal ganglia (TG) were removed under isoflurane anaesthesia. Ten mg of fresh frozen TG (5 samples from 5 animals in each mouse strain) was homogenized with a Mini Beadbeater-1 (BioSpec Products, OK, USA) and subjected to RNA/DNA extraction with AllPrep DNA/RNA Kit (Qiagen, CA, USA). mRNA was reverse transcribed with Random Hexamers (Applied Biosystems, CA, USA). Five replicates were performed in each strain. A 2 µl cDNA aliquot was amplified on the Mx3005 P qPCR system (Agilent Technologies, CA, USA) according to manufacturer’s recommendations with the Taqman gene expression assay for Trpv1 (TRPV1 gene; Mm01246302_m1) and Piezo2 (Fam38b; Piezo2 gene; Mm01265861_m1), which did not detect residual genomic DNA (Applied Biosystems). Mouse ACTB (Applied Biosystems) was used as the endogenous control. The delta-delta CT method was used to quantify relative expression. The assays were carried out in duplicate and the respective relative amounts of Trpv1 and Piezo2 to ACTB were calculated in each sample.

Ono K., Viet C.T., Ye Y., Dang D., Hitomi S., Toyono T., Inenaga K., Dolan J.C, & Schmidt B.L. (2017). Cutaneous pigmentation modulates skin sensitivity via tyrosinase-dependent dopaminergic signalling. Scientific Reports, 7, 9181.

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Quantification of Transcription Factors in Cells

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Total RNA was isolated with the use of the mirVana miRNA kit (Applied Biosystems/ Ambion); cDNA was synthesized with the Taqman Reverse Transcription Kit (Applied Biosystems). Quantitative PCR was performed with an ABI PRISM 7500 sequence detection system with site-specific primers and probes (Applied Biosystems). The comparative threshold cycle method and an internal control (β-actin) were used to normalize the expression of the target genes. List of primers and probes from Applied Biosystems: mouse ACTB, 4352341E; Bcl6, Mm01342164_m1; Tbx21, Mm00450960_m1; Maf, Mm02581355-s1; Irf-4, Mm00516431-m1; Batf, Mm00479410-m1.

Nakayamada S., Poholek A.C., Lu K.T., Takahashi H., Kato M., Iwata S., Hirahara K., Cannons J.L., Schwartzberg P.L., Vahedi G., Sun H.W., Kanno Y, & O’Shea J.J. (2014). Type I Interferon induces binding of STAT1 to Bcl6: Divergent Roles of STAT-family transcription factors in the TFH cell genetic program. Journal of immunology (Baltimore, Md. : 1950), 192(5), 2156-2166.

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